Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15482 and 15483 are separated by 637 nucleotides15483 and 15484 are separated by 513 nucleotides15484 and 15485 are separated by 763 nucleotides15485 and 15486 are separated by 307 nucleotides mRNA_7114: 15482 - K00383 GSR, gor glutathione reductase (NADPH), at 913,071 to 914,998 15482 mRNA_7115: 15483 - K00297 metF, MTHFR methylenetetrahydrofolate reductase (NADPH), at 915,636 to 918,075 15483 mRNA_7116: 15484 - K04393 CDC42 cell division control protein 42, at 918,589 to 919,535 15484 mRNA_7117: 15485 - K12868 SYF2 pre-mRNA-splicing factor SYF2, at 920,299 to 921,602 15485 mRNA_7118: 15486 - SUPERFAMILY--SSF47954, at 921,910 to 924,528 15486
Group Condition mRNA_7114 mRNA_7115 mRNA_7116 mRNA_7117 mRNA_7118
15482 15483 15484 15485 15486
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -4.7 -3.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -4.4 -1.7 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -4.0 -2.0 N.D. N.D.
growth on oleic acid YNB Glucose N.D. -4.9 +0.4 N.D. N.D.
growth on oleic acid YNB Oleic Acid N.D. -3.5 -1.0 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -3.4 -0.8 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -4.7 +0.7 N.D. N.D.
growth on ricinoleic acid YNB Glucose N.D. -4.3 +0.4 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -4.6 +1.0 N.D. N.D.
auxotrophy YNB Glucose N.D. -4.2 +0.6 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -4.4 +0.9 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -3.3 +0.3 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.5 -3.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -3.6 +1.2 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 -0.7 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -2.8 +2.7 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 -0.2 N.D. N.D.
growth on ypd YPD N.D. -0.0 +0.1 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 +0.6 N.D. N.D.
auxotrophy Dropout Complete N.D. -0.2 +1.0 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. -0.0 +1.1 N.D. N.D.
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