Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15484 and 15485 are separated by 763 nucleotides15485 and 15486 are separated by 307 nucleotides15486 and 15487 are separated by 651 nucleotides15487 and 15488 are separated by 196 nucleotides mRNA_7116: 15484 - K04393 CDC42 cell division control protein 42, at 918,589 to 919,535 15484 mRNA_7117: 15485 - K12868 SYF2 pre-mRNA-splicing factor SYF2, at 920,299 to 921,602 15485 mRNA_7118: 15486 - SUPERFAMILY--SSF47954, at 921,910 to 924,528 15486 mRNA_7119: 15487 - ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257, at 925,180 to 925,476 15487 mRNA_7120: 15488 - K01649 leuA 2-isopropylmalate synthase, at 925,673 to 928,023 15488
Group Condition mRNA_7116 mRNA_7117 mRNA_7118 mRNA_7119 mRNA_7120
15484 15485 15486 15487 15488
ypd buoyancy 40 hrs No Buoyancy Separation -3.0 N.D. N.D. +0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -3.3 N.D. N.D. +0.6 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -1.7 N.D. N.D. +0.3 N.D.
growth on oleic acid YNB Oleic Acid -1.0 N.D. N.D. -0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -2.0 N.D. N.D. +0.8 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.8 N.D. N.D. -0.5 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.7 N.D. N.D. +0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.3 N.D. N.D. -0.6 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. N.D. +0.1 N.D.
auxotrophy YNB Glucose +0.6 N.D. N.D. -0.5 N.D.
growth on oleic acid YNB Glucose +0.4 N.D. N.D. -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.7 N.D. N.D. -0.4 N.D.
growth on ypd YPD +0.1 N.D. N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.9 N.D. N.D. -0.4 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.6 N.D. N.D. -0.1 N.D.
auxotrophy Dropout Complete +1.0 N.D. N.D. -0.5 N.D.
growth on ricinoleic acid YNB Glucose +0.4 N.D. N.D. +0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine +1.1 N.D. N.D. -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +1.0 N.D. N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.2 N.D. N.D. +0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +2.7 N.D. N.D. -0.3 N.D.
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