Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15485 and 15486 are separated by 307 nucleotides15486 and 15487 are separated by 651 nucleotides15487 and 15488 are separated by 196 nucleotides15488 and 15489 are separated by 394 nucleotides mRNA_7117: 15485 - K12868 SYF2 pre-mRNA-splicing factor SYF2, at 920,299 to 921,602 15485 mRNA_7118: 15486 - SUPERFAMILY--SSF47954, at 921,910 to 924,528 15486 mRNA_7119: 15487 - ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257, at 925,180 to 925,476 15487 mRNA_7120: 15488 - K01649 leuA 2-isopropylmalate synthase, at 925,673 to 928,023 15488 mRNA_7121: 15489 - PRINTS-Proline rich extensin signature-PR01217,ProSiteProfiles-EH domain profile.-PS50031, at 928,418 to 929,954 15489
Group Condition mRNA_7117 mRNA_7118 mRNA_7119 mRNA_7120 mRNA_7121
15485 15486 15487 15488 15489
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.3 N.D. -1.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.6 N.D. -0.5
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.5 N.D. -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.4 N.D. -0.3
growth on oleic acid YNB Glucose N.D. N.D. -0.2 N.D. -0.3
auxotrophy YNB Glucose N.D. N.D. -0.5 N.D. +0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.3 N.D. +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.4 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.3 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.3 N.D. -0.4
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.1 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.2 N.D. -0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 N.D. -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.2 N.D. -0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.2 N.D. -0.2
auxotrophy Dropout Complete N.D. N.D. -0.5 N.D. +0.7
growth on ypd YPD N.D. N.D. +0.3 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.3 N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.3 N.D. +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.8 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.6 N.D. -0.0
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