Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15487 and 15488 are separated by 196 nucleotides15488 and 15489 are separated by 394 nucleotides15489 and 15490 are separated by 422 nucleotides15490 and 15491 are separated by 147 nucleotides mRNA_7119: 15487 - ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257, at 925,180 to 925,476 15487 mRNA_7120: 15488 - K01649 leuA 2-isopropylmalate synthase, at 925,673 to 928,023 15488 mRNA_7121: 15489 - PRINTS-Proline rich extensin signature-PR01217,ProSiteProfiles-EH domain profile.-PS50031, at 928,418 to 929,954 15489 mRNA_7122: 15490 - K14791 PWP1 periodic tryptophan protein 1, at 930,377 to 932,524 15490 mRNA_7123: 15491 - K00683 QPCT glutaminyl-peptide cyclotransferase, at 932,672 to 934,092 15491
Group Condition mRNA_7119 mRNA_7120 mRNA_7121 mRNA_7122 mRNA_7123
15487 15488 15489 15490 15491
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. -1.1 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.6 N.D. -0.5 N.D. -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 N.D. -0.3 N.D. -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.5 N.D. -0.3 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. -0.4 N.D. -0.7
auxotrophy YNB Glucose -0.5 N.D. +0.1 N.D. -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. -0.4 N.D. -0.4
growth on oleic acid YNB Glucose -0.2 N.D. -0.3 N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine -0.4 N.D. +0.1 N.D. -0.2
growth on ricinoleic acid YNB Glucose +0.2 N.D. -0.2 N.D. -0.4
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. +0.0 N.D. -0.0
auxotrophy Dropout Complete -0.5 N.D. +0.7 N.D. -0.4
growth on oleic acid YNB Oleic Acid -0.3 N.D. +0.0 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. -0.3 N.D. +0.1
growth on ypd YPD +0.3 N.D. -0.1 N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. -0.2 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. -0.0 N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.6 N.D. -0.0 N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.8 N.D. -0.2 N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. +0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. +0.2 N.D. +0.3
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