Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15488 and 15489 are separated by 394 nucleotides15489 and 15490 are separated by 422 nucleotides15490 and 15491 are separated by 147 nucleotides15491 and 15492 are separated by 248 nucleotides mRNA_7120: 15488 - K01649 leuA 2-isopropylmalate synthase, at 925,673 to 928,023 15488 mRNA_7121: 15489 - PRINTS-Proline rich extensin signature-PR01217,ProSiteProfiles-EH domain profile.-PS50031, at 928,418 to 929,954 15489 mRNA_7122: 15490 - K14791 PWP1 periodic tryptophan protein 1, at 930,377 to 932,524 15490 mRNA_7123: 15491 - K00683 QPCT glutaminyl-peptide cyclotransferase, at 932,672 to 934,092 15491 mRNA_7124: 15492 - K02872 RP-L13Ae, RPL13A large subunit ribosomal protein L13Ae, at 934,341 to 935,492 15492
Group Condition mRNA_7120 mRNA_7121 mRNA_7122 mRNA_7123 mRNA_7124
15488 15489 15490 15491 15492
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.1 N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.4 N.D. -0.7 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.5 N.D. -0.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.4 N.D. -0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.3 N.D. -0.4 N.D.
growth on ricinoleic acid YNB Glucose N.D. -0.2 N.D. -0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.3 N.D. -0.2 N.D.
auxotrophy YNB Glucose N.D. +0.1 N.D. -0.4 N.D.
growth on oleic acid YNB Glucose N.D. -0.3 N.D. -0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 N.D. -0.1 N.D.
growth on ypd YPD N.D. -0.1 N.D. -0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.0 N.D. -0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. -0.3 N.D. +0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.2 N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 N.D. -0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 N.D. -0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 N.D. +0.1 N.D.
growth on oleic acid YNB Oleic Acid N.D. +0.0 N.D. +0.1 N.D.
auxotrophy Dropout Complete N.D. +0.7 N.D. -0.4 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 N.D. +0.3 N.D.
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