Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15503 and 15504 are separated by 236 nucleotides15504 and 15505 are separated by 184 nucleotides15505 and 15506 are separated by 224 nucleotides15506 and 15507 are separated by 152 nucleotides mRNA_7135: 15503 - K02948 RP-S11, MRPS11, rpsK small subunit ribosomal protein S11, at 960,089 to 960,921 15503 mRNA_7136: 15504 - K01880 GARS, glyS1 glycyl-tRNA synthetase, at 961,158 to 963,860 15504 mRNA_7137: 15505 - KOG1933 Cholesterol transport protein (Niemann-Pick C disease protein), at 964,045 to 968,474 15505 mRNA_7138: 15506 - K14655 RIB2, PUS8 tRNA pseudouridine synthase 8 / 2,5-diamino-6-(5-phospho-D-ribitylamino)-pyrimidin-4(3H)-one deaminase, at 968,699 to 969,330 15506 mRNA_7139: 15507 - K15272 SLC35A1_2_3 solute carrier family 35 (UDP-sugar transporter), member A1/2/3, at 969,483 to 971,578 15507
Group Condition mRNA_7135 mRNA_7136 mRNA_7137 mRNA_7138 mRNA_7139
15503 15504 15505 15506 15507
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -3.2 N.D. +0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -2.9 N.D. +0.7
growth on ricinoleic acid YNB Glucose N.D. N.D. -1.4 N.D. +0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.1 N.D. -0.4
auxotrophy YNB Glucose N.D. N.D. -1.0 N.D. +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.3 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.8 N.D. +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.4 N.D. +0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.3 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.6 N.D. +0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.2 N.D. +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.1 N.D. -0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.2 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.2 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.1 N.D. -0.0
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.3 N.D. +0.3
growth on ypd YPD N.D. N.D. +0.0 N.D. +0.1
growth on oleic acid YNB Glucose N.D. N.D. -0.2 N.D. +0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.1 N.D. +0.2
auxotrophy Dropout Complete N.D. N.D. -0.2 N.D. +0.7
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.1 N.D. +0.5
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