Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15506 and 15507 are separated by 152 nucleotides15507 and 15508 are separated by 335 nucleotides15508 and 15509 are separated by 110 nucleotides15509 and 15510 are separated by 117 nucleotides mRNA_7138: 15506 - K14655 RIB2, PUS8 tRNA pseudouridine synthase 8 / 2,5-diamino-6-(5-phospho-D-ribitylamino)-pyrimidin-4(3H)-one deaminase, at 968,699 to 969,330 15506 mRNA_7139: 15507 - K15272 SLC35A1_2_3 solute carrier family 35 (UDP-sugar transporter), member A1/2/3, at 969,483 to 971,578 15507 mRNA_7140: 15508 - KOG1029 Endocytic adaptor protein intersectin, at 971,914 to 976,417 15508 mRNA_7141: 15509 - BLAST AP-4 complex subunit epsilon [Leucoagaricus sp. SymC...., at 976,528 to 979,510 15509 mRNA_7142: 15510 - HMMPfam-Domain of unknown function DUF221-PF02714,HMMPfam-Late exocytosis, associated with Golgi transport-PF13967, at 979,628 to 983,826 15510
Group Condition mRNA_7138 mRNA_7139 mRNA_7140 mRNA_7141 mRNA_7142
15506 15507 15508 15509 15510
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.0 -0.4 -0.4 +0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.0 +0.2 -0.1 -0.4
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 -0.2 +0.1 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.4 -0.1 +0.5 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 +0.0 -0.1 -0.0
growth on oleic acid YNB Glucose N.D. +0.3 -0.0 +0.0 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.3 +0.2 -0.1 -0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 +0.1 +0.1 -0.0
growth on ypd YPD N.D. +0.1 -0.0 +0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.4 +0.0 +0.2 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.0 +0.3 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 +0.2 +0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.7 -0.0 +0.4 -0.6
auxotrophy YNB Glucose 75mM Arginine N.D. +0.4 +0.2 -0.1 +0.0
auxotrophy Dropout Complete N.D. +0.7 +0.0 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.0 +0.2 +0.2 +0.3
auxotrophy YNB Glucose N.D. +0.6 +0.2 -0.0 -0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.5 +0.2 -0.1 +0.1
growth on ricinoleic acid YNB Glucose N.D. +0.4 +0.0 +0.6 -0.2
growth on oleic acid YNB Oleic Acid N.D. +0.3 +0.2 +0.1 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 +0.4 +0.1 +0.5
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