Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15515 and 15516 are separated by 941 nucleotides15516 and 15517 are separated by 134 nucleotides15517 and 15518 are separated by 220 nucleotides15518 and 15519 are separated by 194 nucleotides mRNA_7147: 15515 - Hypothetical Protein, at 994,364 to 995,021 15515 mRNA_7148: 15516 - KOG2246 Galactosyltransferases, at 995,963 to 997,965 15516 mRNA_7149: 15517 - K12733 PPIL1 peptidyl-prolyl cis-trans isomerase-like 1, at 998,100 to 998,920 15517 mRNA_7150: 15518 - K01874 MARS, metG methionyl-tRNA synthetase, at 999,141 to 1,001,312 15518 mRNA_7151: 15519 - BLAST putative transmembrane protein [Rhizoctonia solani 123E], at 1,001,507 to 1,002,023 15519
Group Condition mRNA_7147 mRNA_7148 mRNA_7149 mRNA_7150 mRNA_7151
15515 15516 15517 15518 15519
facs on lipid accumulation Sorted Low BODIPY Gate -1.5 -0.9 +0.2 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.7 -0.4 +0.2 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.5 -0.4 +0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.5 +0.0 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.5 -0.1 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.5 -0.0 N.D. N.D.
growth on ricinoleic acid YNB Glucose -0.3 -0.2 +0.1 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.2 +0.0 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.1 -0.0 N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.0 -0.1 +0.1 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 -0.2 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.4 +0.3 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.0 +0.2 N.D. N.D.
growth on ypd YPD -0.3 +0.3 +0.1 N.D. N.D.
growth on oleic acid YNB Glucose -0.1 -0.0 +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.1 +0.2 N.D. N.D.
auxotrophy Dropout Complete +0.1 +0.0 +0.3 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.8 -0.2 -0.3 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.0 +0.2 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.0 +0.4 N.D. N.D.
auxotrophy YNB Glucose -0.0 +0.5 +0.3 N.D. N.D.
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