Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15516 and 15517 are separated by 134 nucleotides15517 and 15518 are separated by 220 nucleotides15518 and 15519 are separated by 194 nucleotides15519 and 15520 are separated by 700 nucleotides mRNA_7148: 15516 - KOG2246 Galactosyltransferases, at 995,963 to 997,965 15516 mRNA_7149: 15517 - K12733 PPIL1 peptidyl-prolyl cis-trans isomerase-like 1, at 998,100 to 998,920 15517 mRNA_7150: 15518 - K01874 MARS, metG methionyl-tRNA synthetase, at 999,141 to 1,001,312 15518 mRNA_7151: 15519 - BLAST putative transmembrane protein [Rhizoctonia solani 123E], at 1,001,507 to 1,002,023 15519 mRNA_7152: 15520 - Hypothetical Protein, at 1,002,724 to 1,003,659 15520
Group Condition mRNA_7148 mRNA_7149 mRNA_7150 mRNA_7151 mRNA_7152
15516 15517 15518 15519 15520
facs on lipid accumulation Sorted High BODIPY Gate -0.4 +0.2 N.D. N.D. -1.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 +0.3 N.D. N.D. -0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.5 -0.0 N.D. N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 +0.0 N.D. N.D. -0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -0.3 N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.0 N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.2 N.D. N.D. -0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.1 N.D. N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.4 +0.2 N.D. N.D. +0.1
growth on oleic acid YNB Oleic Acid -0.1 +0.1 N.D. N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.1 N.D. N.D. +0.0
growth on ricinoleic acid YNB Glucose -0.2 +0.1 N.D. N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.2 N.D. N.D. -0.1
auxotrophy Dropout Complete +0.0 +0.3 N.D. N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.0 N.D. N.D. +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 +0.2 N.D. N.D. +0.2
auxotrophy YNB Glucose +0.5 +0.3 N.D. N.D. -0.4
growth on ypd YPD +0.3 +0.1 N.D. N.D. +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.9 +0.2 N.D. N.D. +1.2
growth on oleic acid YNB Glucose -0.0 +0.3 N.D. N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.4 N.D. N.D. +0.3
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