Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15517 and 15518 are separated by 220 nucleotides15518 and 15519 are separated by 194 nucleotides15519 and 15520 are separated by 700 nucleotides15520 and 15521 are separated by 384 nucleotides mRNA_7149: 15517 - K12733 PPIL1 peptidyl-prolyl cis-trans isomerase-like 1, at 998,100 to 998,920 15517 mRNA_7150: 15518 - K01874 MARS, metG methionyl-tRNA synthetase, at 999,141 to 1,001,312 15518 mRNA_7151: 15519 - BLAST putative transmembrane protein [Rhizoctonia solani 123E], at 1,001,507 to 1,002,023 15519 mRNA_7152: 15520 - Hypothetical Protein, at 1,002,724 to 1,003,659 15520 mRNA_7153: 15521 - Hypothetical Protein, at 1,004,044 to 1,005,448 15521
Group Condition mRNA_7149 mRNA_7150 mRNA_7151 mRNA_7152 mRNA_7153
15517 15518 15519 15520 15521
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. N.D. -1.2 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. N.D. -0.1 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. N.D. -0.9 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. N.D. -0.4 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. N.D. -0.4 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. N.D. -0.3 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. N.D. -0.4 +0.1
growth on oleic acid YNB Oleic Acid +0.1 N.D. N.D. +0.1 -0.1
auxotrophy YNB Glucose +0.3 N.D. N.D. -0.4 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. N.D. -0.1 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. N.D. -0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. N.D. +0.0 +0.1
auxotrophy Dropout Complete +0.3 N.D. N.D. -0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. N.D. +0.4 -0.2
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. +0.1 +0.0
growth on ypd YPD +0.1 N.D. N.D. +0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. N.D. +0.2 +0.0
growth on oleic acid YNB Glucose +0.3 N.D. N.D. +0.3 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.4 N.D. N.D. +0.3 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. N.D. +0.1 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. N.D. +1.2 +1.8
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