Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15518 and 15519 are separated by 194 nucleotides15519 and 15520 are separated by 700 nucleotides15520 and 15521 are separated by 384 nucleotides15521 and 15522 are separated by 170 nucleotides mRNA_7150: 15518 - K01874 MARS, metG methionyl-tRNA synthetase, at 999,141 to 1,001,312 15518 mRNA_7151: 15519 - BLAST putative transmembrane protein [Rhizoctonia solani 123E], at 1,001,507 to 1,002,023 15519 mRNA_7152: 15520 - Hypothetical Protein, at 1,002,724 to 1,003,659 15520 mRNA_7153: 15521 - Hypothetical Protein, at 1,004,044 to 1,005,448 15521 mRNA_7154: 15522 - Hypothetical Protein, at 1,005,619 to 1,007,584 15522
Group Condition mRNA_7150 mRNA_7151 mRNA_7152 mRNA_7153 mRNA_7154
15518 15519 15520 15521 15522
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -1.2 -0.4 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.4 -0.1 -0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.9 +0.1 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.4 -0.0 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.4 +0.1 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.1 +0.2 -0.9
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.2 +0.0 -0.9
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.1 -0.1 -0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 -0.0 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.3 +0.1 -0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.1 +0.0 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.1 -0.3 +0.3
growth on oleic acid YNB Glucose N.D. N.D. +0.3 -0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.3 +0.1 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.0 +0.1 -0.0
auxotrophy YNB Glucose N.D. N.D. -0.4 +0.1 +0.3
auxotrophy Dropout Complete N.D. N.D. -0.1 +0.1 +0.2
growth on ypd YPD N.D. N.D. +0.2 +0.1 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.4 -0.2 -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.1 +0.5 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +1.2 +1.8 +0.6
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