Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15530 and 15531 are separated by 175 nucleotides15531 and 15532 are separated by 141 nucleotides15532 and 15533 are separated by 114 nucleotides15533 and 15534 are separated by 152 nucleotides mRNA_7162: 15530 - ProSiteProfiles-Zinc finger C3H1-type profile.-PS50103, at 1,025,200 to 1,027,264 15530 mRNA_7163: 15531 - Hypothetical Protein, at 1,027,440 to 1,028,287 15531 mRNA_7164: 15532 - K13117 DHX35 ATP-dependent RNA helicase DDX35, at 1,028,429 to 1,031,367 15532 mRNA_7165: 15533 - K01867 WARS, trpS tryptophanyl-tRNA synthetase, at 1,031,482 to 1,033,004 15533 mRNA_7166: 15534 - K01626 E2.5.1.54, aroF, aroG, aroH 3-deoxy-7-phosphoheptulonate synthase, at 1,033,157 to 1,035,237 15534
Group Condition mRNA_7162 mRNA_7163 mRNA_7164 mRNA_7165 mRNA_7166
15530 15531 15532 15533 15534
ypd buoyancy 40 hrs No Buoyancy Separation -1.1 -0.5 -0.2 N.D. +0.2
growth on oleic acid YNB Glucose -0.3 -0.6 -0.2 N.D. +0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 -0.1 -0.2 N.D. -0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.5 -0.3 -0.3 N.D. +0.1
growth on oleic acid YNB Oleic Acid -0.1 -0.4 -0.2 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 -0.2 -0.1 N.D. +0.2
growth on ypd YPD -0.2 -0.1 -0.2 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.3 +0.1 N.D. -0.4
growth on ricinoleic acid YNB Glucose -0.2 +0.0 +0.1 N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.3 -0.0 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 -0.1 +0.1 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.2 -0.1 N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.3 -0.0 N.D. -0.1
auxotrophy Dropout Complete +0.2 -0.1 -0.1 N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.2 +0.1 N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.1 +0.2 N.D. -0.3
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.2 -0.0 N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.0 +0.1 N.D. +0.4
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.0 +0.1 N.D. +0.3
auxotrophy YNB Glucose +0.3 +0.2 -0.1 N.D. +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 -0.1 +0.2 N.D. +0.2
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