Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15533 and 15534 are separated by 152 nucleotides15534 and 15535 are separated by 177 nucleotides15535 and 15536 are separated by 192 nucleotides15536 and 15537 are separated by 256 nucleotides mRNA_7165: 15533 - K01867 WARS, trpS tryptophanyl-tRNA synthetase, at 1,031,482 to 1,033,004 15533 mRNA_7166: 15534 - K01626 E2.5.1.54, aroF, aroG, aroH 3-deoxy-7-phosphoheptulonate synthase, at 1,033,157 to 1,035,237 15534 mRNA_7167: 15535 - ProSiteProfiles-Zinc finger C3H1-type profile.-PS50103, at 1,035,415 to 1,036,383 15535 mRNA_7168: 15536 - HMMPfam-WD domain, G-beta repeat-PF00400,HMMPfam-PFU (PLAA family ubiquitin binding)-PF09070,PRINTS-G protein beta WD-40 repeat signature-PR00320,ProSiteProfiles-Trp-Asp (WD) repeats profile.-PS50082,ProSiteProfiles-Trp-Asp (WD) repeats circular profile.-PS50294,ProSiteProfiles-PFU domain profile.-PS51394,SMART-WD40 repeats-SM00320,SUPERFAMILY--SSF50978, at 1,036,576 to 1,038,409 15536 mRNA_7169: 15537 - K13137 STRAP, UNRIP serine-threonine kinase receptor-associated protein, at 1,038,666 to 1,040,206 15537
Group Condition mRNA_7165 mRNA_7166 mRNA_7167 mRNA_7168 mRNA_7169
15533 15534 15535 15536 15537
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 -0.7 +0.1 -0.7
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 +0.1 -0.2 -1.6
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.3 -0.1 +0.1 -1.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 +0.1 -0.0 -1.6
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 -0.2 -0.1 -1.2
growth on ricinoleic acid YNB Glucose N.D. -0.3 -0.4 +0.0 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 +0.0 -0.1 -1.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.9 -0.5 +0.1 +0.5
growth on ypd YPD N.D. -0.1 +0.1 +0.1 -0.9
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 -0.2 -0.2 -0.6
ypd buoyancy 40 hrs High Buoyancy N.D. +0.3 -0.2 -0.1 -0.6
growth on oleic acid YNB Glucose N.D. +0.0 -0.3 -0.1 +0.2
growth on oleic acid YNB Oleic Acid N.D. -0.2 -0.3 -0.1 +0.7
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.4 -0.2 +0.2 +0.8
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.4 +0.1 -0.0 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 +0.0 -0.0 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 +0.4 -0.0 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 +0.0 -0.1 +1.2
auxotrophy Dropout Complete N.D. -0.1 +0.0 -0.1 +1.3
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 +0.0 -0.2 +1.2
auxotrophy YNB Glucose N.D. +0.2 +0.1 +0.0 +1.0
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