Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15540 and 15541 are separated by 320 nucleotides15541 and 15542 are separated by 392 nucleotides15542 and 15543 are separated by 419 nucleotides15543 and 15544 are separated by 220 nucleotides mRNA_7172: 15540 - K01408 IDE, ide insulysin, at 1,045,325 to 1,049,371 15540 mRNA_7173: 15541 - K02891 RP-L22e, RPL22 large subunit ribosomal protein L22e, at 1,049,692 to 1,050,738 15541 mRNA_7174: 15542 - K11131 DKC1, NOLA4, CBF5 H/ACA ribonucleoprotein complex subunit 4, at 1,051,131 to 1,053,156 15542 mRNA_7175: 15543 - K12272 SRPRB, SRP102 signal recognition particle receptor subunit beta, at 1,053,576 to 1,055,087 15543 mRNA_7176: 15544 - KOG4635 Vacuolar import and degradation protein, at 1,055,308 to 1,056,885 15544
Group Condition mRNA_7172 mRNA_7173 mRNA_7174 mRNA_7175 mRNA_7176
15540 15541 15542 15543 15544
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 N.D. N.D. N.D. -0.5
growth on oleic acid YNB Oleic Acid -0.1 N.D. N.D. N.D. -0.6
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. N.D. N.D. -0.4
growth on ricinoleic acid YNB Glucose -0.0 N.D. N.D. N.D. -0.6
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. N.D. N.D. -0.4
growth on oleic acid YNB Glucose -0.0 N.D. N.D. N.D. -0.6
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. N.D. N.D. -0.5
auxotrophy Dropout Complete -0.3 N.D. N.D. N.D. -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. N.D. N.D. -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. N.D. N.D. -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. N.D. N.D. -0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. N.D. N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. N.D. N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine +0.0 N.D. N.D. N.D. -0.0
auxotrophy YNB Glucose +0.1 N.D. N.D. N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. N.D. N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. N.D. N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy +0.4 N.D. N.D. N.D. +0.1
growth on ypd YPD +0.1 N.D. N.D. N.D. +0.5
ypd buoyancy 40 hrs No Buoyancy Separation +0.6 N.D. N.D. N.D. +0.2
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