Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15541 and 15542 are separated by 392 nucleotides15542 and 15543 are separated by 419 nucleotides15543 and 15544 are separated by 220 nucleotides15544 and 15545 are separated by 284 nucleotides mRNA_7173: 15541 - K02891 RP-L22e, RPL22 large subunit ribosomal protein L22e, at 1,049,692 to 1,050,738 15541 mRNA_7174: 15542 - K11131 DKC1, NOLA4, CBF5 H/ACA ribonucleoprotein complex subunit 4, at 1,051,131 to 1,053,156 15542 mRNA_7175: 15543 - K12272 SRPRB, SRP102 signal recognition particle receptor subunit beta, at 1,053,576 to 1,055,087 15543 mRNA_7176: 15544 - KOG4635 Vacuolar import and degradation protein, at 1,055,308 to 1,056,885 15544 mRNA_7177: 15545 - KOG0565 Inositol polyphosphate 5-phosphatase and related proteins, at 1,057,170 to 1,058,665 15545
Group Condition mRNA_7173 mRNA_7174 mRNA_7175 mRNA_7176 mRNA_7177
15541 15542 15543 15544 15545
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.5 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. -0.5 -0.4
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. -0.4 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. -0.5 -0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. -0.6 -0.0
auxotrophy Dropout Complete N.D. N.D. N.D. -0.2 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.4 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.4 -0.0
growth on oleic acid YNB Glucose N.D. N.D. N.D. -0.6 +0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -0.6 +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. -0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. -0.0 -0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -0.2 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. -0.1 +0.1
auxotrophy YNB Glucose N.D. N.D. N.D. -0.1 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. -0.4 +0.5
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. -0.2 +0.4
growth on ypd YPD N.D. N.D. N.D. +0.5 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. -0.0 +0.6
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