Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15542 and 15543 are separated by 419 nucleotides15543 and 15544 are separated by 220 nucleotides15544 and 15545 are separated by 284 nucleotides15545 and 15546 are separated by 172 nucleotides mRNA_7174: 15542 - K11131 DKC1, NOLA4, CBF5 H/ACA ribonucleoprotein complex subunit 4, at 1,051,131 to 1,053,156 15542 mRNA_7175: 15543 - K12272 SRPRB, SRP102 signal recognition particle receptor subunit beta, at 1,053,576 to 1,055,087 15543 mRNA_7176: 15544 - KOG4635 Vacuolar import and degradation protein, at 1,055,308 to 1,056,885 15544 mRNA_7177: 15545 - KOG0565 Inositol polyphosphate 5-phosphatase and related proteins, at 1,057,170 to 1,058,665 15545 mRNA_7178: 15546 - KOG0417 Ubiquitin-protein ligase, at 1,058,838 to 1,059,935 15546
Group Condition mRNA_7174 mRNA_7175 mRNA_7176 mRNA_7177 mRNA_7178
15542 15543 15544 15545 15546
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.4 -0.1 -4.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.2 +0.1 -2.6
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.5 -0.4 -1.4
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.4 -0.4 -1.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.2 -0.2 -2.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.5 -0.2 -1.4
auxotrophy Dropout Complete N.D. N.D. -0.2 -0.4 -0.9
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.5 -0.4 -0.6
growth on oleic acid YNB Glucose N.D. N.D. -0.6 +0.2 -0.8
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.0 -0.3 -0.8
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.6 -0.0 -0.4
auxotrophy YNB Glucose N.D. N.D. -0.1 +0.2 -1.0
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.1 +0.1 -0.9
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.4 -0.0 -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.1 +0.1 -0.8
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.6 +0.2 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.2 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.0 +0.6 -0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.2 +0.4 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.4 +0.5 +0.1
growth on ypd YPD N.D. N.D. +0.5 -0.0 +0.4
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