Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15543 and 15544 are separated by 220 nucleotides15544 and 15545 are separated by 284 nucleotides15545 and 15546 are separated by 172 nucleotides15546 and 15547 are separated by 404 nucleotides mRNA_7175: 15543 - K12272 SRPRB, SRP102 signal recognition particle receptor subunit beta, at 1,053,576 to 1,055,087 15543 mRNA_7176: 15544 - KOG4635 Vacuolar import and degradation protein, at 1,055,308 to 1,056,885 15544 mRNA_7177: 15545 - KOG0565 Inositol polyphosphate 5-phosphatase and related proteins, at 1,057,170 to 1,058,665 15545 mRNA_7178: 15546 - KOG0417 Ubiquitin-protein ligase, at 1,058,838 to 1,059,935 15546 mRNA_7179: 15547 - Hypothetical Protein, at 1,060,340 to 1,061,881 15547
Group Condition mRNA_7175 mRNA_7176 mRNA_7177 mRNA_7178 mRNA_7179
15543 15544 15545 15546 15547
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.4 -0.1 -4.0 +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 +0.1 -2.6 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 -0.2 -2.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.5 -0.2 -1.4 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.4 -0.4 -1.4 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.5 -0.4 -1.4 +0.5
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.5 -0.4 -0.6 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.4 -0.0 -0.2 -0.7
auxotrophy Dropout Complete N.D. -0.2 -0.4 -0.9 +0.2
growth on oleic acid YNB Glucose N.D. -0.6 +0.2 -0.8 +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 -0.1 -0.2 -0.6
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 +0.1 -0.9 -0.0
growth on ricinoleic acid YNB Glucose N.D. -0.6 -0.0 -0.4 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 +0.1 -0.8 -0.2
auxotrophy YNB Glucose N.D. -0.1 +0.2 -1.0 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.0 +0.6 -0.8 -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. -0.0 -0.3 -0.8 +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 +0.4 -0.4 -0.3
growth on oleic acid YNB Oleic Acid N.D. -0.6 +0.2 -0.2 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.4 +0.5 +0.1 -0.2
growth on ypd YPD N.D. +0.5 -0.0 +0.4 -0.0
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