Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15552 and 15553 are separated by 140 nucleotides15553 and 15554 are separated by 670 nucleotides15554 and 15555 are separated by 1287 nucleotides15555 and 15556 are separated by 309 nucleotides mRNA_7184: 15552 - K02219 CKS1 cyclin-dependent kinase regulatory subunit CKS1, at 1,072,312 to 1,073,593 15552 mRNA_7185: 15553 - K07300 chaA, CAX Ca2+-H+ antiporter, at 1,073,734 to 1,075,504 15553 mRNA_7186: 15554 - HMMPfam-alpha/beta hydrolase fold-PF07859,SUPERFAMILY--SSF53474, at 1,076,175 to 1,077,434 15554 mRNA_7187: 15555 - K00799 GST, gst glutathione S-transferase, at 1,078,722 to 1,079,084 15555 mRNA_7188: 15556 - K01870 IARS, ileS isoleucyl-tRNA synthetase, at 1,079,394 to 1,083,450 15556
Group Condition mRNA_7184 mRNA_7185 mRNA_7186 mRNA_7187 mRNA_7188
15552 15553 15554 15555 15556
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -1.2 -0.3 -0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -1.1 -0.2 -0.3 N.D.
auxotrophy Dropout Complete +0.0 -0.2 -0.6 -0.7 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.4 -1.1 +0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 -0.4 -0.3 +0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.2 +0.0 -0.7 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 +0.3 -0.3 -0.5 N.D.
auxotrophy YNB Glucose +0.2 -0.3 -0.2 -0.5 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.3 -0.6 -0.2 N.D.
growth on oleic acid YNB Oleic Acid -0.1 -0.0 -0.1 -0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.4 -0.6 -0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 -0.3 -0.1 +0.7 N.D.
growth on oleic acid YNB Glucose -0.1 -0.2 -0.2 +0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.4 -0.3 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.1 -0.7 +0.5 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.2 -0.3 +0.7 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 +0.1 +0.2 +0.3 N.D.
growth on ricinoleic acid YNB Glucose -0.1 +0.1 -0.0 +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +0.2 -0.6 +0.9 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.4 -0.2 +0.7 N.D.
growth on ypd YPD +0.6 +0.0 -0.0 +0.6 N.D.
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