Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15553 and 15554 are separated by 670 nucleotides15554 and 15555 are separated by 1287 nucleotides15555 and 15556 are separated by 309 nucleotides15556 and 15557 are separated by 306 nucleotides mRNA_7185: 15553 - K07300 chaA, CAX Ca2+-H+ antiporter, at 1,073,734 to 1,075,504 15553 mRNA_7186: 15554 - HMMPfam-alpha/beta hydrolase fold-PF07859,SUPERFAMILY--SSF53474, at 1,076,175 to 1,077,434 15554 mRNA_7187: 15555 - K00799 GST, gst glutathione S-transferase, at 1,078,722 to 1,079,084 15555 mRNA_7188: 15556 - K01870 IARS, ileS isoleucyl-tRNA synthetase, at 1,079,394 to 1,083,450 15556 mRNA_7189: 15557 - K14847 RPF2 ribosome production factor 2, at 1,083,757 to 1,085,072 15557
Group Condition mRNA_7185 mRNA_7186 mRNA_7187 mRNA_7188 mRNA_7189
15553 15554 15555 15556 15557
facs on lipid accumulation Sorted No BODIPY Gate -1.2 -0.3 -0.1 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -1.1 -0.2 -0.3 N.D. N.D.
auxotrophy Dropout Complete -0.2 -0.6 -0.7 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 -1.1 +0.3 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.0 -0.7 N.D. N.D.
auxotrophy YNB Glucose -0.3 -0.2 -0.5 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.3 -0.6 -0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 -0.3 -0.5 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 -0.3 +0.2 N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.0 -0.1 -0.3 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.4 -0.6 -0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.7 +0.5 N.D. N.D.
growth on oleic acid YNB Glucose -0.2 -0.2 +0.2 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.4 -0.3 -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.3 +0.7 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -0.1 +0.7 N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.1 -0.0 +0.3 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.6 +0.9 N.D. N.D.
growth on ypd YPD +0.0 -0.0 +0.6 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.2 +0.3 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.4 -0.2 +0.7 N.D. N.D.
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