Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15555 and 15556 are separated by 309 nucleotides15556 and 15557 are separated by 306 nucleotides15557 and 15558 are separated by 265 nucleotides15558 and 15559 are separated by 356 nucleotides mRNA_7187: 15555 - K00799 GST, gst glutathione S-transferase, at 1,078,722 to 1,079,084 15555 mRNA_7188: 15556 - K01870 IARS, ileS isoleucyl-tRNA synthetase, at 1,079,394 to 1,083,450 15556 mRNA_7189: 15557 - K14847 RPF2 ribosome production factor 2, at 1,083,757 to 1,085,072 15557 mRNA_7190: 15558 - Hypothetical Protein, at 1,085,338 to 1,086,300 15558 mRNA_7191: 15559 - Hypothetical Protein, at 1,086,657 to 1,088,494 15559
Group Condition mRNA_7187 mRNA_7188 mRNA_7189 mRNA_7190 mRNA_7191
15555 15556 15557 15558 15559
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. N.D. -1.0 -0.3
auxotrophy Dropout Complete -0.7 N.D. N.D. -0.2 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 N.D. N.D. -0.5 -0.9
growth on oleic acid YNB Glucose +0.2 N.D. N.D. -0.6 -0.7
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. N.D. -0.6 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 N.D. N.D. +0.3 -0.7
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. N.D. -0.2 -0.8
auxotrophy YNB Glucose 75mM Arginine -0.7 N.D. N.D. -0.3 +0.3
auxotrophy YNB Glucose -0.5 N.D. N.D. -0.3 +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. N.D. -0.3 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.7 N.D. N.D. -0.6 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. N.D. -0.2 +0.3
growth on ypd YPD +0.6 N.D. N.D. +0.0 -0.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 N.D. N.D. -0.3 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. N.D. +0.0 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.9 N.D. N.D. +0.8 -1.0
ypd buoyancy 40 hrs High Buoyancy +0.7 N.D. N.D. -0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. N.D. +0.1 +0.4
growth on oleic acid YNB Oleic Acid -0.3 N.D. N.D. +0.5 +1.0
growth on ricinoleic acid YNB Glucose +0.3 N.D. N.D. +0.3 +0.9
growth on ricinoleic acid YNB Ricinoleic Acid +0.7 N.D. N.D. +0.5 +0.8
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