Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15579 and 15580 are separated by 458 nucleotides15580 and 15581 are separated by 309 nucleotides15581 and 15582 are separated by 137 nucleotides15582 and 15583 are separated by 314 nucleotides mRNA_7211: 15579 - HMMPfam-Fungal specific transcription factor domain-PF11951,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,SUPERFAMILY--SSF57701, at 1,140,051 to 1,142,038 15579 mRNA_7212: 15580 - KOG3764 Vesicular amine transporter, at 1,142,497 to 1,144,513 15580 mRNA_7213: 15581 - K14636 SLC18A3, VACHT MFS transporter, DHA1 family, solute carrier family 18 (vesicular acetylcholine transporter), member 3, at 1,144,823 to 1,146,874 15581 mRNA_7214: 15582 - K17792 TIM54 mitochondrial import inner membrane translocase subunit TIM54, at 1,147,012 to 1,148,920 15582 mRNA_7215: 15583 - K10253 K10253 DOPA 4,5-dioxygenase, at 1,149,235 to 1,150,219 15583
Group Condition mRNA_7211 mRNA_7212 mRNA_7213 mRNA_7214 mRNA_7215
15579 15580 15581 15582 15583
growth on oleic acid YNB Oleic Acid -0.1 +0.3 -0.4 N.D. -0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.0 +0.3 -0.3 N.D. -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 -0.1 +0.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 +0.1 -0.3 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.0 -0.2 N.D. +0.1
growth on ypd YPD -0.2 +0.1 -0.3 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.1 -0.1 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.1 +0.0 N.D. -0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 +0.1 +0.0 N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine +0.4 -0.0 -0.0 N.D. -0.4
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.0 -0.2 N.D. +0.0
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.0 +0.1 N.D. -0.1
growth on oleic acid YNB Glucose -0.2 +0.2 -0.1 N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.1 +0.2 N.D. -0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.0 +0.3 +0.4 N.D. -0.5
auxotrophy Dropout Complete +0.3 +0.1 -0.1 N.D. +0.0
growth on ricinoleic acid YNB Glucose +0.1 +0.3 +0.3 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.0 +0.4 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 +0.3 +0.0 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.2 -0.1 N.D. +0.3
auxotrophy YNB Glucose +0.4 +0.1 -0.1 N.D. +0.1
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