Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15581 and 15582 are separated by 137 nucleotides15582 and 15583 are separated by 314 nucleotides15583 and 15584 are separated by 209 nucleotides15584 and 15585 are separated by 549 nucleotides mRNA_7213: 15581 - K14636 SLC18A3, VACHT MFS transporter, DHA1 family, solute carrier family 18 (vesicular acetylcholine transporter), member 3, at 1,144,823 to 1,146,874 15581 mRNA_7214: 15582 - K17792 TIM54 mitochondrial import inner membrane translocase subunit TIM54, at 1,147,012 to 1,148,920 15582 mRNA_7215: 15583 - K10253 K10253 DOPA 4,5-dioxygenase, at 1,149,235 to 1,150,219 15583 mRNA_7216: 15584 - K01535 PMA1, PMA2 H+-transporting ATPase, at 1,150,429 to 1,154,847 15584 mRNA_7217: 15585 - KOG3309 Ferredoxin, at 1,155,397 to 1,156,419 15585
Group Condition mRNA_7213 mRNA_7214 mRNA_7215 mRNA_7216 mRNA_7217
15581 15582 15583 15584 15585
growth on oleic acid YNB Oleic Acid -0.4 N.D. -0.3 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 N.D. -0.1 -0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. -0.3 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. +0.1 -0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 N.D. -0.2 -0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. -0.4 +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.4 N.D. -0.5 -0.1 N.D.
growth on ypd YPD -0.3 N.D. +0.2 -0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. -0.2 +0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. +0.0 +0.2 N.D.
growth on oleic acid YNB Glucose -0.1 N.D. +0.0 +0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. -0.1 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. +0.1 -0.2 N.D.
auxotrophy Dropout Complete -0.1 N.D. +0.0 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. +0.3 -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. -0.0 +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. +0.1 +0.1 N.D.
auxotrophy YNB Glucose -0.1 N.D. +0.1 +0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. -0.0 +0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.3 N.D. -0.1 +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. -0.1 +0.1 N.D.
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