Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15582 and 15583 are separated by 314 nucleotides15583 and 15584 are separated by 209 nucleotides15584 and 15585 are separated by 549 nucleotides15585 and 15586 are separated by 191 nucleotides mRNA_7214: 15582 - K17792 TIM54 mitochondrial import inner membrane translocase subunit TIM54, at 1,147,012 to 1,148,920 15582 mRNA_7215: 15583 - K10253 K10253 DOPA 4,5-dioxygenase, at 1,149,235 to 1,150,219 15583 mRNA_7216: 15584 - K01535 PMA1, PMA2 H+-transporting ATPase, at 1,150,429 to 1,154,847 15584 mRNA_7217: 15585 - KOG3309 Ferredoxin, at 1,155,397 to 1,156,419 15585 mRNA_7218: 15586 - K20405 NPRL2 nitrogen permease regulator 2-like protein, at 1,156,611 to 1,158,564 15586
Group Condition mRNA_7214 mRNA_7215 mRNA_7216 mRNA_7217 mRNA_7218
15582 15583 15584 15585 15586
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 +0.1 N.D. -1.5
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.5 -0.1 N.D. -0.9
auxotrophy YNB Glucose 75mM Methionine N.D. -0.4 +0.1 N.D. -0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 -0.2 N.D. -0.9
growth on oleic acid YNB Glucose N.D. +0.0 +0.0 N.D. -1.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 -0.2 N.D. -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 -0.2 N.D. -0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 -0.2 N.D. -0.5
growth on oleic acid YNB Oleic Acid N.D. -0.3 +0.1 N.D. -0.6
auxotrophy Dropout Complete N.D. +0.0 +0.1 N.D. -0.7
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.2 +0.1 N.D. -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 +0.1 N.D. -0.2
growth on ricinoleic acid YNB Glucose N.D. -0.1 +0.2 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.3 -0.1 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 +0.1 N.D. -0.1
auxotrophy YNB Glucose N.D. +0.1 +0.2 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 +0.1 N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 +0.1 N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.0 +0.1 N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.0 +0.2 N.D. +0.2
growth on ypd YPD N.D. +0.2 -0.1 N.D. +1.7
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