Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15583 and 15584 are separated by 209 nucleotides15584 and 15585 are separated by 549 nucleotides15585 and 15586 are separated by 191 nucleotides15586 and 15587 are separated by 87 nucleotides mRNA_7215: 15583 - K10253 K10253 DOPA 4,5-dioxygenase, at 1,149,235 to 1,150,219 15583 mRNA_7216: 15584 - K01535 PMA1, PMA2 H+-transporting ATPase, at 1,150,429 to 1,154,847 15584 mRNA_7217: 15585 - KOG3309 Ferredoxin, at 1,155,397 to 1,156,419 15585 mRNA_7218: 15586 - K20405 NPRL2 nitrogen permease regulator 2-like protein, at 1,156,611 to 1,158,564 15586 mRNA_7219: 15587 - K15053 CHMP7 charged multivesicular body protein 7, at 1,158,652 to 1,160,281 15587
Group Condition mRNA_7215 mRNA_7216 mRNA_7217 mRNA_7218 mRNA_7219
15583 15584 15585 15586 15587
facs on lipid accumulation Sorted No BODIPY Gate -0.3 +0.1 N.D. -1.5 -1.9
facs on lipid accumulation Sorted High BODIPY Gate -0.5 -0.1 N.D. -0.9 -1.3
auxotrophy Dropout Complete +0.0 +0.1 N.D. -0.7 -2.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.1 N.D. -0.4 -1.9
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 +0.2 N.D. +0.2 -2.7
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.2 N.D. -0.5 -1.2
growth on oleic acid YNB Glucose +0.0 +0.0 N.D. -1.1 -0.8
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.1 N.D. -0.2 -1.1
growth on oleic acid YNB Oleic Acid -0.3 +0.1 N.D. -0.6 -0.5
growth on ricinoleic acid YNB Glucose -0.1 +0.2 N.D. -0.2 -1.0
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.1 N.D. +0.2 -1.4
auxotrophy YNB Glucose 75mM Methionine -0.4 +0.1 N.D. -0.9 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.1 N.D. +0.2 -1.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.1 N.D. +0.2 -1.1
auxotrophy YNB Glucose +0.1 +0.2 N.D. -0.1 -0.8
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.1 N.D. -0.1 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.2 N.D. -0.7 +1.8
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.2 N.D. -0.5 +1.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.2 N.D. -0.9 +2.7
growth on ypd YPD +0.2 -0.1 N.D. +1.7 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.1 N.D. -0.1 +2.4
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