Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15588 and 15589 are separated by 1208 nucleotides15589 and 15590 are separated by 349 nucleotides15590 and 15591 are separated by 273 nucleotides15591 and 15592 are separated by 136 nucleotides mRNA_7220: 15588 - HMMPfam-zinc-RING finger domain-PF14634,ProSitePatterns-Zinc finger RING-type signature.-PS00518,ProSiteProfiles-Zinc finger RING-type profile.-PS50089,SUPERFAMILY--SSF57850, at 1,160,377 to 1,161,832 15588 mRNA_7221: 15589 - K02133 ATPeF1B, ATP5B, ATP2 F-type H+-transporting ATPase subunit beta, at 1,163,041 to 1,165,362 15589 mRNA_7222: 15590 - KOG1572 Predicted protein tyrosine phosphatase, at 1,165,712 to 1,166,653 15590 mRNA_7223: 15591 - K19983 EXOC1, SEC3 exocyst complex component 1, at 1,166,927 to 1,171,458 15591 mRNA_7224: 15592 - K15564 CTK2 CTD kinase subunit beta, at 1,171,595 to 1,173,622 15592
Group Condition mRNA_7220 mRNA_7221 mRNA_7222 mRNA_7223 mRNA_7224
15588 15589 15590 15591 15592
facs on lipid accumulation Sorted High BODIPY Gate -1.0 N.D. +0.0 -2.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.3 N.D. +0.1 -2.4 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.9 N.D. +0.5 -1.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. +0.0 -1.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. +0.1 -1.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.5 N.D. -0.3 +0.1 N.D.
growth on oleic acid YNB Oleic Acid +0.0 N.D. -0.4 -0.2 N.D.
growth on oleic acid YNB Glucose +0.2 N.D. -0.4 -0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 N.D. +0.1 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 N.D. -0.2 -0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.6 N.D. -0.3 -0.4 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. +0.1 -0.2 N.D.
auxotrophy YNB Glucose -0.1 N.D. +0.1 +0.0 N.D.
growth on ricinoleic acid YNB Glucose +0.4 N.D. -0.2 -0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. +0.0 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 N.D. -0.0 -0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. +0.1 -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.2 +0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 N.D. -0.2 +0.2 N.D.
auxotrophy Dropout Complete +0.5 N.D. +0.5 +0.2 N.D.
growth on ypd YPD +0.5 N.D. +0.4 +0.4 N.D.
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