Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15590 and 15591 are separated by 273 nucleotides15591 and 15592 are separated by 136 nucleotides15592 and 15593 are separated by 736 nucleotides15593 and 15594 are separated by 582 nucleotides mRNA_7222: 15590 - KOG1572 Predicted protein tyrosine phosphatase, at 1,165,712 to 1,166,653 15590 mRNA_7223: 15591 - K19983 EXOC1, SEC3 exocyst complex component 1, at 1,166,927 to 1,171,458 15591 mRNA_7224: 15592 - K15564 CTK2 CTD kinase subunit beta, at 1,171,595 to 1,173,622 15592 mRNA_7225: 15593 - Hypothetical Protein, at 1,174,359 to 1,174,926 15593 mRNA_7226: 15594 - HMMPfam-Demethylmenaquinone methyltransferase-PF03737,SUPERFAMILY--SSF89562, at 1,175,509 to 1,176,564 15594
Group Condition mRNA_7222 mRNA_7223 mRNA_7224 mRNA_7225 mRNA_7226
15590 15591 15592 15593 15594
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -2.4 N.D. +1.2 -0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 -0.3 N.D. +0.1 -0.7
growth on oleic acid YNB Glucose -0.4 -0.4 N.D. +0.2 -0.3
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.1 N.D. -0.0 -0.7
growth on oleic acid YNB Oleic Acid -0.4 -0.2 N.D. -0.0 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.2 N.D. -0.4 -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -2.1 N.D. +0.9 +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.4 N.D. +0.4 -0.3
auxotrophy YNB Glucose +0.1 +0.0 N.D. +0.0 -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 -1.0 N.D. +0.6 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.3 N.D. +0.4 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -1.1 N.D. +0.7 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.2 N.D. +0.6 -0.5
ypd buoyancy 40 hrs Low Buoyancy -0.3 +0.1 N.D. +0.5 -0.1
growth on ricinoleic acid YNB Glucose -0.2 -0.1 N.D. +0.5 +0.2
auxotrophy Dropout Complete +0.5 +0.2 N.D. +0.1 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 -1.0 N.D. +0.8 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.0 N.D. +0.2 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.1 N.D. +0.7 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.2 N.D. +0.5 +0.2
growth on ypd YPD +0.4 +0.4 N.D. +0.8 -0.6
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