Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15595 and 15596 are separated by 609 nucleotides15596 and 15597 are separated by 434 nucleotides15597 and 15598 are separated by 240 nucleotides15598 and 15599 are separated by 186 nucleotides mRNA_7227: 15595 - K03239 EIF2B1 translation initiation factor eIF-2B subunit alpha, at 1,176,800 to 1,178,543 15595 mRNA_7228: 15596 - KOG1674 Cyclin, at 1,179,153 to 1,180,280 15596 mRNA_7229: 15597 - Hypothetical Protein, at 1,180,715 to 1,181,387 15597 mRNA_7230: 15598 - K07019 K07019 uncharacterized protein, at 1,181,628 to 1,183,243 15598 mRNA_7231: 15599 - Hypothetical Protein, at 1,183,430 to 1,185,124 15599
Group Condition mRNA_7227 mRNA_7228 mRNA_7229 mRNA_7230 mRNA_7231
15595 15596 15597 15598 15599
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 +0.0 -0.3 -3.0 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.9 -0.5 -2.2 +0.2 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.2 -1.1 -0.2 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -1.3 +0.0 +1.0 -0.7 -0.2
growth on oleic acid YNB Oleic Acid -0.7 -0.1 -0.6 +0.2 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.2 +0.4 -0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.5 -1.4 +0.3 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.1 -0.0 -0.2 -0.1
growth on oleic acid YNB Glucose +0.1 -0.1 -0.1 +0.2 +0.2
growth on ricinoleic acid YNB Glucose +0.7 +0.1 +0.5 -0.5 +0.0
growth on ypd YPD +0.0 +0.4 +0.3 -0.0 +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.1 +0.8 -0.1 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.3 -0.2 +0.8 +0.1 +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.2 +0.8 +0.3 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 +0.3 +1.9 -0.3 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.3 +0.0 +0.6 +0.3 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.9 +0.8 +2.2 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.9 +0.7 +2.7 +0.2 -0.6
auxotrophy Dropout Complete +0.3 +0.6 +1.0 +0.3 +0.3
auxotrophy YNB Glucose +0.6 +0.5 +1.5 +0.5 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +0.7 +3.6 -0.5 -0.2
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