Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15612 and 15613 are separated by 432 nucleotides15613 and 15614 are separated by 72 nucleotides15614 and 15615 are separated by 146 nucleotides15615 and 15616 are separated by 192 nucleotides mRNA_7244: 15612 - HMMPfam-PQ loop repeat-PF04193,SMART-Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins.-SM00679, at 1,209,765 to 1,211,262 15612 mRNA_7245: 15613 - K17972 NAA20, NAT3 N-terminal acetyltransferase B complex catalytic subunit, at 1,211,695 to 1,212,788 15613 mRNA_7246: 15614 - Hypothetical Protein, at 1,212,861 to 1,214,455 15614 mRNA_7247: 15615 - Hypothetical Protein, at 1,214,602 to 1,216,220 15615 mRNA_7248: 15616 - HMMPfam-N-acetyltransferase-PF00797,SUPERFAMILY--SSF54001, at 1,216,413 to 1,217,523 15616
Group Condition mRNA_7244 mRNA_7245 mRNA_7246 mRNA_7247 mRNA_7248
15612 15613 15614 15615 15616
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. -0.1 -0.5 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 N.D. -0.3 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. -0.2 +0.1 -0.1
growth on ricinoleic acid YNB Glucose +0.2 N.D. +0.2 -0.3 -0.0
growth on oleic acid YNB Oleic Acid +0.1 N.D. +0.1 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. +0.0 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 N.D. -0.1 +0.1 -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.1 -0.1 +0.1
auxotrophy Dropout Complete +0.4 N.D. +0.3 -0.2 -0.3
auxotrophy YNB Glucose 75mM Arginine +0.4 N.D. +0.0 -0.1 -0.1
growth on oleic acid YNB Glucose +0.1 N.D. +0.0 +0.1 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. +0.1 +0.2 -0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.1 +0.2 +0.0
auxotrophy YNB Glucose 75mM Methionine +0.4 N.D. +0.3 -0.0 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 N.D. +0.1 +0.3 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. +0.5 +0.3 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. +0.3 -0.0 +0.1
auxotrophy YNB Glucose +0.4 N.D. +0.4 -0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. +0.5 +0.1 +0.1
growth on ypd YPD +0.3 N.D. +0.2 +0.2 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 N.D. +0.2 +0.3 +0.1
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