Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15631 and 15632 are separated by 222 nucleotides15632 and 15633 are separated by 609 nucleotides15633 and 15634 are separated by 3001 nucleotides15634 and 15635 are separated by 181 nucleotides mRNA_7263: 15631 - K00615 E2.2.1.1, tktA, tktB transketolase, at 1,251,489 to 1,254,335 15631 mRNA_7264: 15632 - Hypothetical Protein, at 1,254,558 to 1,258,564 15632 mRNA_7265: 15633 - Hypothetical Protein, at 1,259,174 to 1,259,392 15633 mRNA_7266: 15634 - Hypothetical Protein, at 1,262,394 to 1,262,742 15634 mRNA_7267: 15635 - Hypothetical Protein, at 1,262,924 to 1,263,981 15635
Group Condition mRNA_7263 mRNA_7264 mRNA_7265 mRNA_7266 mRNA_7267
15631 15632 15633 15634 15635
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 N.D. -2.7 +0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 N.D. -3.1 +0.6
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 N.D. -2.1 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 N.D. -0.6 -0.7
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 N.D. -0.5 -0.4
auxotrophy YNB Glucose 75mM Methionine N.D. -0.0 N.D. +0.0 -0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.0 N.D. -0.1 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 N.D. -0.5 -0.2
growth on ricinoleic acid YNB Glucose N.D. +0.2 N.D. -0.3 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 N.D. -0.1 -0.4
auxotrophy YNB Glucose N.D. +0.2 N.D. +0.5 -1.0
auxotrophy Dropout Complete N.D. -0.1 N.D. +0.4 -0.6
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 N.D. +0.2 -0.5
growth on oleic acid YNB Glucose N.D. +0.0 N.D. +0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 N.D. -0.0 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.2 N.D. +0.3 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 N.D. +0.0 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.0 N.D. +0.8 -0.1
growth on oleic acid YNB Oleic Acid N.D. -0.1 N.D. +1.1 -0.2
growth on ypd YPD N.D. -0.3 N.D. +1.0 +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 N.D. +0.7 +0.4
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