Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15642 and 15643 are separated by 671 nucleotides15643 and 15644 are separated by 85 nucleotides15644 and 15645 are separated by 272 nucleotides15645 and 15646 are separated by 235 nucleotides mRNA_7274: 15642 - Hypothetical Protein, at 1,275,952 to 1,277,293 15642 mRNA_7275: 15643 - HMMPfam-Uncharacterized conserved protein (DUF2346)-PF09803, at 1,277,965 to 1,278,426 15643 mRNA_7276: 15644 - K17806 PAM17, TIM23 mitochondrial import inner membrane translocase subunit TIM23, at 1,278,512 to 1,279,383 15644 mRNA_7277: 15645 - HMMPfam-Glycosyl hydrolases family 16-PF00722,SUPERFAMILY--SSF49899, at 1,279,656 to 1,282,236 15645 mRNA_7278: 15646 - HMMPfam-Fungal specific transcription factor domain-PF04082,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 1,282,472 to 1,285,388 15646
Group Condition mRNA_7274 mRNA_7275 mRNA_7276 mRNA_7277 mRNA_7278
15642 15643 15644 15645 15646
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -1.3 -0.5 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.4 -0.0 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.4 +0.1 -0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.3 +0.0 -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 -0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.0 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.5 -0.0 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.0 -0.0 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.2 -0.1 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.4 +0.3 +0.0
growth on oleic acid YNB Glucose N.D. N.D. -0.1 +0.0 +0.0
growth on ypd YPD N.D. N.D. -0.1 +0.1 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.1 +0.0 +0.1
auxotrophy YNB Glucose N.D. N.D. -0.1 +0.3 +0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.3 +0.3 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.1 +0.1 +0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.1 +0.2 -0.0
auxotrophy Dropout Complete N.D. N.D. +0.2 +0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.1 +0.4 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.1 +0.2 +0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.0 +0.4 +0.8
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