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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
15674 and 15675 are separated by 140 nucleotides
15675 and 15676 overlap by 37 nucleotides
15676 and 15677 are separated by 655 nucleotides
15677 and 15678 are separated by 1291 nucleotides
mRNA_7306: 15674 - KOG1217 Fibrillins and related proteins containing Ca2+-binding EGF-like domains, at 1,356,547 to 1,358,907
15674
mRNA_7307: 15675 - Hypothetical Protein, at 1,359,048 to 1,360,624
15675
mRNA_7308: 15676 - Hypothetical Protein, at 1,360,588 to 1,362,752
15676
mRNA_7309: 15677 - KOG0803 Predicted E3 ubiquitin ligase, at 1,363,408 to 1,369,017
15677
mRNA_7310: 15678 - BLAST cytochrome P450 3A4 [Neurospora crassa OR74A], at 1,370,309 to 1,371,999
15678
Group
Condition
mRNA
_7306
mRNA
_7307
mRNA
_7308
mRNA
_7309
mRNA
_7310
15674
15675
15676
15677
15678
facs on lipid accumulation
Sorted High BODIPY Gate
-0.2
-0.3
-0.4
-0.8
-0.1
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.2
-0.5
-0.6
+0.0
-0.0
auxotrophy
Dropout Complete
+0.1
-0.5
-0.4
-0.0
+0.1
auxotrophy
YNB Glucose 75mM Arginine
-0.0
-0.3
-0.4
-0.0
+0.2
growth on ricinoleic acid
YNB Glucose
+0.4
-0.3
-0.4
-0.2
-0.1
auxotrophy
YNB Glucose 75mM Methionine
+0.1
-0.6
-0.2
+0.0
+0.0
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.4
-0.6
-0.3
+0.0
+0.1
growth on ypd
YPD
+0.3
-0.9
-0.0
+0.2
+0.1
auxotrophy
YNB Glucose
+0.2
-0.5
-0.3
+0.1
+0.3
facs on lipid accumulation
Sorted No BODIPY Gate
-0.4
+0.3
+0.2
-0.2
+0.1
growth on oleic acid
YNB Oleic Acid
+0.3
-0.7
+0.0
+0.4
+0.0
ypd buoyancy
40 hrs No Buoyancy Separation
+0.1
+0.1
-0.0
+0.3
-0.3
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.9
+0.4
+0.6
+0.2
-0.0
growth on oleic acid
YNB Glucose
+0.4
-0.2
+0.2
-0.1
+0.1
ypd buoyancy
40 hrs Median Buoyancy
+0.3
+0.1
+0.1
-0.1
-0.0
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.4
+0.1
+0.4
-0.4
+0.1
ypd buoyancy
40 hrs High Buoyancy
+0.1
+0.3
+0.0
+0.1
-0.0
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.3
-0.3
+0.4
+0.0
+0.1
ypd buoyancy
40 hrs Low Buoyancy
+0.4
+0.3
+0.3
-0.1
-0.1
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.3
+0.5
+0.4
-0.1
+0.3
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.3
+0.4
+1.1
+0.7
+0.2
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