Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15676 and 15677 are separated by 655 nucleotides15677 and 15678 are separated by 1291 nucleotides15678 and 15679 are separated by 652 nucleotides15679 and 15680 are separated by 390 nucleotides mRNA_7308: 15676 - Hypothetical Protein, at 1,360,588 to 1,362,752 15676 mRNA_7309: 15677 - KOG0803 Predicted E3 ubiquitin ligase, at 1,363,408 to 1,369,017 15677 mRNA_7310: 15678 - BLAST cytochrome P450 3A4 [Neurospora crassa OR74A], at 1,370,309 to 1,371,999 15678 mRNA_7311: 15679 - K00940 ndk, NME nucleoside-diphosphate kinase, at 1,372,652 to 1,373,595 15679 mRNA_7312: 15680 - HMMPfam-Protein of unknown function (DUF3429)-PF11911, at 1,373,986 to 1,375,689 15680
Group Condition mRNA_7308 mRNA_7309 mRNA_7310 mRNA_7311 mRNA_7312
15676 15677 15678 15679 15680
facs on lipid accumulation Sorted High BODIPY Gate -0.4 -0.8 -0.1 N.D. -0.3
growth on ricinoleic acid YNB Glucose -0.4 -0.2 -0.1 N.D. -0.1
auxotrophy Dropout Complete -0.4 -0.0 +0.1 N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine -0.4 -0.0 +0.2 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 -0.4 +0.1 N.D. -0.3
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.0 +0.0 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 +0.0 -0.0 N.D. +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 +0.0 +0.1 N.D. +0.0
auxotrophy YNB Glucose -0.3 +0.1 +0.3 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.2 +0.1 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.1 -0.0 N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.1 -0.0 N.D. -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.1 -0.1 N.D. +0.0
growth on oleic acid YNB Glucose +0.2 -0.1 +0.1 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +0.3 -0.3 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 +0.0 +0.1 N.D. -0.2
growth on ypd YPD -0.0 +0.2 +0.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 -0.1 +0.3 N.D. -0.1
growth on oleic acid YNB Oleic Acid +0.0 +0.4 +0.0 N.D. +0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.6 +0.2 -0.0 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.1 +0.7 +0.2 N.D. +0.1
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