Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15678 and 15679 are separated by 652 nucleotides15679 and 15680 are separated by 390 nucleotides15680 and 15681 are separated by 395 nucleotides15681 and 15682 are separated by 301 nucleotides mRNA_7310: 15678 - BLAST cytochrome P450 3A4 [Neurospora crassa OR74A], at 1,370,309 to 1,371,999 15678 mRNA_7311: 15679 - K00940 ndk, NME nucleoside-diphosphate kinase, at 1,372,652 to 1,373,595 15679 mRNA_7312: 15680 - HMMPfam-Protein of unknown function (DUF3429)-PF11911, at 1,373,986 to 1,375,689 15680 mRNA_7313: 15681 - K00417 QCR7, UQCRB ubiquinol-cytochrome c reductase subunit 7, at 1,376,085 to 1,377,067 15681 mRNA_7314: 15682 - Hypothetical Protein, at 1,377,369 to 1,379,605 15682
Group Condition mRNA_7310 mRNA_7311 mRNA_7312 mRNA_7313 mRNA_7314
15678 15679 15680 15681 15682
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. +0.2 N.D. -0.9
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 N.D. +0.3 N.D. -1.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -0.1 N.D. -0.9
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. -0.3 N.D. -0.5
growth on ricinoleic acid YNB Glucose -0.1 N.D. -0.1 N.D. -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. +0.0 N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. -0.2 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. -0.3 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. -0.0 N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. -0.0 N.D. -0.0
growth on ypd YPD +0.1 N.D. +0.1 N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. -0.2 N.D. +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 N.D. +0.1 N.D. -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. -0.1 N.D. +0.0
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. -0.2 N.D. +0.2
growth on oleic acid YNB Glucose +0.1 N.D. -0.1 N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. +0.0 N.D. +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 N.D. +0.1 N.D. +0.2
growth on oleic acid YNB Oleic Acid +0.0 N.D. +0.0 N.D. +0.6
auxotrophy Dropout Complete +0.1 N.D. -0.3 N.D. +0.9
auxotrophy YNB Glucose +0.3 N.D. -0.1 N.D. +0.6
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