Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15679 and 15680 are separated by 390 nucleotides15680 and 15681 are separated by 395 nucleotides15681 and 15682 are separated by 301 nucleotides15682 and 15683 are separated by 373 nucleotides mRNA_7311: 15679 - K00940 ndk, NME nucleoside-diphosphate kinase, at 1,372,652 to 1,373,595 15679 mRNA_7312: 15680 - HMMPfam-Protein of unknown function (DUF3429)-PF11911, at 1,373,986 to 1,375,689 15680 mRNA_7313: 15681 - K00417 QCR7, UQCRB ubiquinol-cytochrome c reductase subunit 7, at 1,376,085 to 1,377,067 15681 mRNA_7314: 15682 - Hypothetical Protein, at 1,377,369 to 1,379,605 15682 mRNA_7315: 15683 - K05527 bolA BolA protein, at 1,379,979 to 1,380,539 15683
Group Condition mRNA_7311 mRNA_7312 mRNA_7313 mRNA_7314 mRNA_7315
15679 15680 15681 15682 15683
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 N.D. -0.9 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 N.D. -0.9 -0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.3 N.D. -0.5 -0.2
growth on ypd YPD N.D. +0.1 N.D. -0.2 -0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 N.D. -0.1 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.3 N.D. +0.1 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 N.D. +0.0 -0.4
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 N.D. -0.0 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 N.D. -1.1 +0.6
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 N.D. -0.0 -0.1
growth on ricinoleic acid YNB Glucose N.D. -0.1 N.D. -0.2 +0.1
growth on oleic acid YNB Glucose N.D. -0.1 N.D. +0.3 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.0 N.D. -0.4 +0.7
growth on oleic acid YNB Oleic Acid N.D. +0.0 N.D. +0.6 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. -0.2 N.D. +0.2 +0.4
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 N.D. +0.2 +0.7
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 N.D. +0.4 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 N.D. +0.2 +0.8
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 N.D. -0.0 +1.2
auxotrophy Dropout Complete N.D. -0.3 N.D. +0.9 +1.2
auxotrophy YNB Glucose N.D. -0.1 N.D. +0.6 +1.5
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