Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15681 and 15682 are separated by 301 nucleotides15682 and 15683 are separated by 373 nucleotides15683 and 15684 are separated by 11 nucleotides15684 and 15685 are separated by 454 nucleotides mRNA_7313: 15681 - K00417 QCR7, UQCRB ubiquinol-cytochrome c reductase subunit 7, at 1,376,085 to 1,377,067 15681 mRNA_7314: 15682 - Hypothetical Protein, at 1,377,369 to 1,379,605 15682 mRNA_7315: 15683 - K05527 bolA BolA protein, at 1,379,979 to 1,380,539 15683 mRNA_7316: 15684 - KOG4178 Soluble epoxide hydrolase, at 1,380,551 to 1,382,072 15684 mRNA_7317: 15685 - K01652 E2.2.1.6L, ilvB, ilvG, ilvI acetolactate synthase I/II/III large subunit, at 1,382,527 to 1,385,139 15685
Group Condition mRNA_7313 mRNA_7314 mRNA_7315 mRNA_7316 mRNA_7317
15681 15682 15683 15684 15685
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.9 -0.3 -0.4 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -1.1 +0.6 -0.7 N.D.
growth on oleic acid YNB Glucose N.D. +0.3 -0.3 -1.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 -0.5 -0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.5 -0.2 -0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.9 -0.4 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 -0.4 -0.6 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.0 -0.4 -0.5 N.D.
growth on ypd YPD N.D. -0.2 -0.8 +0.1 N.D.
growth on ricinoleic acid YNB Glucose N.D. -0.2 +0.1 -0.6 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 -0.1 -0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 -0.3 +0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 +0.4 -0.8 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 +0.7 -0.9 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.4 +0.7 -0.4 N.D.
growth on oleic acid YNB Oleic Acid N.D. +0.6 -0.1 -0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.0 +1.2 -0.8 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 +0.8 -0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.4 +0.4 +0.1 N.D.
auxotrophy YNB Glucose N.D. +0.6 +1.5 -0.5 N.D.
auxotrophy Dropout Complete N.D. +0.9 +1.2 -0.3 N.D.
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