Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15683 and 15684 are separated by 11 nucleotides15684 and 15685 are separated by 454 nucleotides15685 and 15686 are separated by 335 nucleotides15686 and 15687 are separated by 1325 nucleotides mRNA_7315: 15683 - K05527 bolA BolA protein, at 1,379,979 to 1,380,539 15683 mRNA_7316: 15684 - KOG4178 Soluble epoxide hydrolase, at 1,380,551 to 1,382,072 15684 mRNA_7317: 15685 - K01652 E2.2.1.6L, ilvB, ilvG, ilvI acetolactate synthase I/II/III large subunit, at 1,382,527 to 1,385,139 15685 mRNA_7318: 15686 - KOG4018 Uncharacterized conserved protein, contains RWD domain, at 1,385,475 to 1,386,748 15686 mRNA_7319: 15687 - Hypothetical Protein, at 1,388,074 to 1,389,867 15687
Group Condition mRNA_7315 mRNA_7316 mRNA_7317 mRNA_7318 mRNA_7319
15683 15684 15685 15686 15687
growth on oleic acid YNB Glucose -0.3 -1.1 N.D. -0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.4 N.D. -0.5 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 -0.4 N.D. -0.3 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 -0.5 N.D. -0.3 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 -0.6 N.D. -0.0 +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.3 N.D. -0.2 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.8 N.D. -0.0 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.3 N.D. -0.0 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.8 -0.3 N.D. -0.1 -0.8
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 +0.3 N.D. -0.4 +0.2
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.0 N.D. +0.1 -0.0
growth on ricinoleic acid YNB Glucose +0.1 -0.6 N.D. +0.3 +0.1
growth on oleic acid YNB Oleic Acid -0.1 -0.3 N.D. -0.0 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate +1.2 -0.8 N.D. -0.1 -0.3
growth on ypd YPD -0.8 +0.1 N.D. +0.3 +0.5
auxotrophy YNB Glucose 75mM Methionine +0.7 -0.9 N.D. +0.3 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.4 +0.1 N.D. +0.1 +0.0
auxotrophy Dropout Complete +1.2 -0.3 N.D. +0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.7 -0.4 N.D. +0.5 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.6 -0.7 N.D. +0.5 +0.6
auxotrophy YNB Glucose +1.5 -0.5 N.D. +0.4 -0.1
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