Fitness for 3 genes in Rhodosporidium toruloides IFO0880

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500 nt 15685 and 15686 are separated by 335 nucleotides15686 and 15687 are separated by 1325 nucleotides mRNA_7317: 15685 - K01652 E2.2.1.6L, ilvB, ilvG, ilvI acetolactate synthase I/II/III large subunit, at 1,382,527 to 1,385,139 15685 mRNA_7318: 15686 - KOG4018 Uncharacterized conserved protein, contains RWD domain, at 1,385,475 to 1,386,748 15686 mRNA_7319: 15687 - Hypothetical Protein, at 1,388,074 to 1,389,867 15687
Group Condition mRNA_7317 mRNA_7318 mRNA_7319
15685 15686 15687
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 -0.8
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.5 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.3 +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.0 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.4 +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.3 +0.2
growth on oleic acid YNB Glucose N.D. -0.1 +0.1
auxotrophy Dropout Complete N.D. +0.1 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.0 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 +0.0
auxotrophy YNB Glucose N.D. +0.4 -0.1
growth on oleic acid YNB Oleic Acid N.D. -0.0 +0.4
growth on ricinoleic acid YNB Glucose N.D. +0.3 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.3 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.5 +0.3
growth on ypd YPD N.D. +0.3 +0.5
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.5 +0.6
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