Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15689 and 15690 are separated by 658 nucleotides15690 and 15691 are separated by 706 nucleotides15691 and 15692 are separated by 250 nucleotides15692 and 15693 are separated by 190 nucleotides mRNA_7321: 15689 - KOG1339 Aspartyl protease, at 1,943 to 3,763 15689 mRNA_7322: 15690 - K14849 RRP1 ribosomal RNA-processing protein 1, at 4,422 to 5,823 15690 mRNA_7323: 15691 - K10579 UBE2M, UBC12 ubiquitin-conjugating enzyme E2 M, at 6,530 to 7,758 15691 mRNA_7324: 15692 - K20967 MOCS1 GTP 3',8-cyclase / cyclic pyranopterin monophosphate synthase, at 8,009 to 10,735 15692 mRNA_7325: 15693 - K01640 E4.1.3.4, HMGCL, hmgL hydroxymethylglutaryl-CoA lyase, at 10,926 to 12,277 15693
Group Condition mRNA_7321 mRNA_7322 mRNA_7323 mRNA_7324 mRNA_7325
15689 15690 15691 15692 15693
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. N.D. -0.3 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. N.D. -0.2 -0.1
auxotrophy YNB Glucose -0.1 N.D. N.D. -0.2 +0.1
growth on ricinoleic acid YNB Glucose -0.0 N.D. N.D. -0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. N.D. -0.2 +0.1
auxotrophy Dropout Complete +0.1 N.D. N.D. -0.4 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. N.D. -0.2 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 N.D. N.D. -0.1 +0.4
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. N.D. +0.3 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. N.D. +0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. N.D. +0.2 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. N.D. +0.1 -0.0
growth on oleic acid YNB Oleic Acid +0.0 N.D. N.D. +0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. N.D. -0.2 +0.3
growth on ypd YPD +0.2 N.D. N.D. -0.1 +0.4
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. N.D. +0.3 +0.1
growth on oleic acid YNB Glucose +0.1 N.D. N.D. +0.2 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. N.D. +0.2 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 N.D. N.D. +0.3 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. N.D. +0.1 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. N.D. +0.3 +0.6
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