Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15691 and 15692 are separated by 250 nucleotides15692 and 15693 are separated by 190 nucleotides15693 and 15694 are separated by 207 nucleotides15694 and 15695 are separated by 218 nucleotides mRNA_7323: 15691 - K10579 UBE2M, UBC12 ubiquitin-conjugating enzyme E2 M, at 6,530 to 7,758 15691 mRNA_7324: 15692 - K20967 MOCS1 GTP 3',8-cyclase / cyclic pyranopterin monophosphate synthase, at 8,009 to 10,735 15692 mRNA_7325: 15693 - K01640 E4.1.3.4, HMGCL, hmgL hydroxymethylglutaryl-CoA lyase, at 10,926 to 12,277 15693 mRNA_7326: 15694 - K01969 E6.4.1.4B 3-methylcrotonyl-CoA carboxylase beta subunit, at 12,485 to 14,861 15694 mRNA_7327: 15695 - KOG2883 NIPSNAP1 protein, at 15,080 to 16,504 15695
Group Condition mRNA_7323 mRNA_7324 mRNA_7325 mRNA_7326 mRNA_7327
15691 15692 15693 15694 15695
auxotrophy YNB Glucose 75mM Arginine N.D. -0.3 -0.1 -0.7 +0.1
auxotrophy Dropout Complete N.D. -0.4 +0.3 -0.6 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.3 +0.1 -0.5 -0.6
auxotrophy YNB Glucose N.D. -0.2 +0.1 -0.5 +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 -0.1 -0.4 +0.3
growth on ypd YPD N.D. -0.1 +0.4 -0.2 -0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 -0.0 -0.0 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.3 -0.2 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 +0.2 -0.3 -0.0
growth on ricinoleic acid YNB Glucose N.D. -0.2 +0.1 -0.0 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 +0.4 -0.0 -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 -0.2 -0.0 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 +0.1 -0.1 +0.4
growth on oleic acid YNB Oleic Acid N.D. +0.1 +0.1 -0.1 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.2 +0.3 +0.2 +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 +0.3 +0.2 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.3 +0.2 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.3 +0.6 -0.0 -0.1
growth on oleic acid YNB Glucose N.D. +0.2 +0.3 +0.1 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 +0.5 +0.5 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 +0.5 +0.3 +0.5
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