Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15708 and 15709 are separated by 93 nucleotides15709 and 15710 are separated by 463 nucleotides15710 and 15711 are separated by 209 nucleotides15711 and 15712 are separated by 227 nucleotides mRNA_7340: 15708 - K08073 PNKP bifunctional polynucleotide phosphatase/kinase, at 39,779 to 41,768 15708 mRNA_7341: 15709 - KOG4062 6-O-methylguanine-DNA methyltransferase MGMT/MGT1, involved in DNA repair, at 41,862 to 42,699 15709 mRNA_7342: 15710 - Hypothetical Protein, at 43,163 to 45,525 15710 mRNA_7343: 15711 - K04798 pfdB, PFDN6 prefoldin beta subunit, at 45,735 to 46,573 15711 mRNA_7344: 15712 - K01697 E4.2.1.22, CBS cystathionine beta-synthase, at 46,801 to 48,602 15712
Group Condition mRNA_7340 mRNA_7341 mRNA_7342 mRNA_7343 mRNA_7344
15708 15709 15710 15711 15712
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 -0.1 +0.5 -2.2 -0.6
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.2 +0.2 -0.8 -0.6
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 -0.0 -0.3 -0.1 -1.2
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.2 +0.1 -0.1 -1.4
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.1 +0.2 -0.3 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.6 +0.0 -0.7 -0.7
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.3 +0.5 -0.3 -0.4
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.5 +0.2 +0.6 -0.5
growth on ricinoleic acid YNB Glucose -0.0 +0.6 -0.5 -0.2 -0.2
auxotrophy YNB Glucose +0.2 -0.5 +0.0 +0.5 -0.3
auxotrophy Dropout Complete +0.2 -0.3 -0.0 +0.7 -0.5
growth on ypd YPD -0.0 -0.4 +0.2 +0.3 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.1 +0.7 +0.1 -0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.2 +0.0 -0.4 +0.5
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.2 +0.5 +0.8 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.5 -0.1 +0.1 -0.1
growth on oleic acid YNB Glucose +0.1 -0.1 +0.1 +0.5 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.2 +0.4 +0.8 -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.2 +0.6 +0.2 -0.3
growth on oleic acid YNB Oleic Acid +0.2 +0.1 +0.2 -0.1 +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.5 +0.4 +1.0 -0.7
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