Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15730 and 15731 are separated by 94 nucleotides15731 and 15732 are separated by 397 nucleotides15732 and 15733 are separated by 95 nucleotides15733 and 15734 are separated by 251 nucleotides mRNA_7362: 15730 - K13035 NIT4 beta-cyano-L-alanine hydratase/nitrilase, at 93,946 to 95,192 15730 mRNA_7363: 15731 - SUPERFAMILY--SSF48371, at 95,287 to 98,403 15731 mRNA_7364: 15732 - KOG1337 N-methyltransferase, at 98,801 to 102,847 15732 mRNA_7365: 15733 - Hypothetical Protein, at 102,943 to 104,194 15733 mRNA_7366: 15734 - SUPERFAMILY--SSF57701, at 104,446 to 105,512 15734
Group Condition mRNA_7362 mRNA_7363 mRNA_7364 mRNA_7365 mRNA_7366
15730 15731 15732 15733 15734
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 N.D. -0.4 -0.5 -0.4
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. -0.1 -0.2 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.5 N.D. +0.1 -0.3 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. -0.0 +0.1 -0.4
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. -0.2 -0.1 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. -0.0 -0.0 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. +0.2 -0.2 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. +0.2 -0.2 -0.0
growth on ypd YPD +0.1 N.D. +0.1 +0.3 -0.4
auxotrophy YNB Glucose 75mM Arginine -0.0 N.D. +0.1 -0.2 +0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.0 N.D. +0.1 +0.1 +0.1
auxotrophy YNB Glucose +0.1 N.D. +0.1 -0.3 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 N.D. +0.4 +0.4 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. +0.3 +0.0 +0.4
growth on oleic acid YNB Oleic Acid +0.5 N.D. +0.1 -0.0 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. +0.1 +0.0 +0.3
growth on oleic acid YNB Glucose -0.0 N.D. +0.3 +0.2 +0.2
auxotrophy Dropout Complete -0.0 N.D. +0.3 -0.0 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 N.D. +0.5 -0.0 +0.1
growth on ricinoleic acid YNB Glucose +0.7 N.D. +0.5 -0.3 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.6 N.D. +0.4 +0.1 +0.3
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