Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15740 and 15741 are separated by 532 nucleotides15741 and 15742 are separated by 926 nucleotides15742 and 15743 are separated by 2421 nucleotides15743 and 15744 are separated by 295 nucleotides mRNA_7372: 15740 - K03869 CUL3 cullin 3, at 116,214 to 119,420 15740 mRNA_7373: 15741 - HMMPfam-FAD binding domain-PF01494,PRINTS-Aromatic-ring hydroxylase (flavoprotein monooxygenase) signature-PR00420,SUPERFAMILY--SSF51905, at 119,953 to 122,182 15741 mRNA_7374: 15742 - Hypothetical Protein, at 123,109 to 123,612 15742 mRNA_7375: 15743 - HMMPfam-Chromosome segregation protein Csm1/Pcs1-PF12539, at 126,034 to 127,845 15743 mRNA_7376: 15744 - K19030 PFKFB4 6-phosphofructo-2-kinase / fructose-2,6-biphosphatase 4, at 128,141 to 130,355 15744
Group Condition mRNA_7372 mRNA_7373 mRNA_7374 mRNA_7375 mRNA_7376
15740 15741 15742 15743 15744
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.3 -4.1 -0.1 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.5 -0.2 -0.4 -1.8 -1.0
facs on lipid accumulation Sorted No BODIPY Gate -0.6 +0.4 -0.6 -1.1 -1.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.3 -1.5 +0.1 -0.7
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +0.1 +0.6 -0.2 -1.1
growth on ypd YPD -0.3 -0.4 +0.0 -0.3 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 -0.2 -0.3 -0.3 +0.5
growth on ricinoleic acid YNB Glucose -0.1 +0.3 -0.7 +0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 +0.5 -0.0 -0.1 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 +0.6 -1.0 +0.6 -0.5
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.5 -0.5 +0.1 +0.1
growth on oleic acid YNB Glucose +0.1 +0.4 +0.3 -0.5 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +1.2 -0.2 +0.1 -0.1
auxotrophy YNB Glucose -0.1 +0.2 +1.1 -0.2 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 +0.2 -0.2 +0.6 +0.3
auxotrophy Dropout Complete +0.1 +0.2 +1.1 -0.2 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.0 +0.0 +1.6 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.7 -0.3 +0.5 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.4 +1.0 -0.2 +0.0
growth on oleic acid YNB Oleic Acid -0.1 +0.7 +0.8 -0.6 +0.5
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.3 +0.6 +0.4 +0.1
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