Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15744 and 15745 are separated by 216 nucleotides15745 and 15746 are separated by 1141 nucleotides15746 and 15747 are separated by 1051 nucleotides15747 and 15748 are separated by 228 nucleotides mRNA_7376: 15744 - K19030 PFKFB4 6-phosphofructo-2-kinase / fructose-2,6-biphosphatase 4, at 128,141 to 130,355 15744 mRNA_7377: 15745 - KOG1256 Long-chain acyl-CoA synthetases (AMP-forming), at 130,572 to 131,007 15745 mRNA_7378: 15746 - KOG1180 Acyl-CoA synthetase, at 132,149 to 133,750 15746 mRNA_7379: 15747 - SUPERFAMILY--SSF49344, at 134,802 to 136,760 15747 mRNA_7380: 15748 - K01897 ACSL, fadD long-chain acyl-CoA synthetase, at 136,989 to 140,179 15748
Group Condition mRNA_7376 mRNA_7377 mRNA_7378 mRNA_7379 mRNA_7380
15744 15745 15746 15747 15748
growth on oleic acid YNB Glucose +0.1 -0.1 -0.5 -0.0 -1.6
facs on lipid accumulation Sorted Low BODIPY Gate -1.1 +0.1 +0.4 +0.1 -1.5
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.1 -0.1 -0.3 -1.2
growth on ricinoleic acid YNB Glucose -0.2 -0.1 -0.3 -0.1 -1.2
facs on lipid accumulation Sorted No BODIPY Gate -1.0 -0.2 +0.1 +0.2 -0.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.1 -0.5 +0.2 -1.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.7 -0.2 -0.2 -0.0 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 -0.1 -1.1 -0.3 -0.1
auxotrophy YNB Glucose -0.0 -0.3 +0.2 -0.0 -1.0
facs on lipid accumulation Sorted High BODIPY Gate -1.0 -0.2 +0.8 +0.4 -1.1
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.2 -0.5 -0.2 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.3 -0.8 -0.1 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.4 -0.2 -0.0 +0.0
auxotrophy Dropout Complete +0.1 -0.1 +0.5 -0.3 -0.8
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.1 +1.0 -0.0 -1.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.1 -0.8 +0.2 +0.3
auxotrophy YNB Glucose 75mM Arginine +0.0 +0.0 +0.9 +0.0 -1.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 +0.2 -0.6 -0.1 +1.0
growth on oleic acid YNB Oleic Acid +0.5 -0.1 +0.2 -0.0 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.2 +0.3 +0.0 +0.1
growth on ypd YPD +0.1 +0.1 +1.1 +0.1 +0.0
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