Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15746 and 15747 are separated by 1051 nucleotides15747 and 15748 are separated by 228 nucleotides15748 and 15749 are separated by 1050 nucleotides15749 and 15750 are separated by 47 nucleotides mRNA_7378: 15746 - KOG1180 Acyl-CoA synthetase, at 132,149 to 133,750 15746 mRNA_7379: 15747 - SUPERFAMILY--SSF49344, at 134,802 to 136,760 15747 mRNA_7380: 15748 - K01897 ACSL, fadD long-chain acyl-CoA synthetase, at 136,989 to 140,179 15748 mRNA_7381: 15749 - K03128 TAF2 transcription initiation factor TFIID subunit 2, at 141,230 to 147,514 15749 mRNA_7382: 15750 - Hypothetical Protein, at 147,562 to 148,955 15750
Group Condition mRNA_7378 mRNA_7379 mRNA_7380 mRNA_7381 mRNA_7382
15746 15747 15748 15749 15750
growth on oleic acid YNB Glucose -0.5 -0.0 -1.6 N.D. +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.3 -1.2 N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -1.1 -0.3 -0.1 N.D. +0.1
growth on ricinoleic acid YNB Glucose -0.3 -0.1 -1.2 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 +0.2 -1.2 N.D. +0.4
ypd buoyancy 40 hrs Low Buoyancy -0.5 -0.2 +0.2 N.D. -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.2 -0.8 N.D. -0.3
ypd buoyancy 40 hrs High Buoyancy -0.8 -0.1 +0.4 N.D. -0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 +0.1 -1.5 N.D. +0.2
auxotrophy YNB Glucose +0.2 -0.0 -1.0 N.D. +0.1
auxotrophy Dropout Complete +0.5 -0.3 -0.8 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.8 +0.2 +0.3 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.0 -0.1 N.D. +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.0 +0.0 N.D. +0.0
auxotrophy YNB Glucose 75mM Arginine +0.9 +0.0 -1.3 N.D. +0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.8 +0.4 -1.1 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine +1.0 -0.0 -1.2 N.D. +0.3
growth on oleic acid YNB Oleic Acid +0.2 -0.0 -0.3 N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 -0.1 +1.0 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 +0.0 +0.1 N.D. +0.2
growth on ypd YPD +1.1 +0.1 +0.0 N.D. +0.3
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