Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15747 and 15748 are separated by 228 nucleotides15748 and 15749 are separated by 1050 nucleotides15749 and 15750 are separated by 47 nucleotides15750 and 15751 are separated by 694 nucleotides mRNA_7379: 15747 - SUPERFAMILY--SSF49344, at 134,802 to 136,760 15747 mRNA_7380: 15748 - K01897 ACSL, fadD long-chain acyl-CoA synthetase, at 136,989 to 140,179 15748 mRNA_7381: 15749 - K03128 TAF2 transcription initiation factor TFIID subunit 2, at 141,230 to 147,514 15749 mRNA_7382: 15750 - Hypothetical Protein, at 147,562 to 148,955 15750 mRNA_7383: 15751 - K19355 MAN mannan endo-1,4-beta-mannosidase, at 149,650 to 152,306 15751
Group Condition mRNA_7379 mRNA_7380 mRNA_7381 mRNA_7382 mRNA_7383
15747 15748 15749 15750 15751
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -1.5 N.D. +0.2 -2.0
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.8 N.D. -0.3 -1.1
growth on oleic acid YNB Glucose -0.0 -1.6 N.D. +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -1.2 N.D. +0.4 -0.5
facs on lipid accumulation Sorted High BODIPY Gate +0.4 -1.1 N.D. -0.1 -0.2
auxotrophy YNB Glucose 75mM Methionine -0.0 -1.2 N.D. +0.3 +0.0
auxotrophy YNB Glucose -0.0 -1.0 N.D. +0.1 +0.0
auxotrophy YNB Glucose 75mM Arginine +0.0 -1.3 N.D. +0.3 +0.2
growth on ricinoleic acid YNB Glucose -0.1 -1.2 N.D. +0.1 +0.4
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.4 N.D. -0.3 -0.6
auxotrophy Dropout Complete -0.3 -0.8 N.D. +0.1 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -1.2 N.D. +0.1 +1.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 -0.1 N.D. +0.0 +0.0
growth on oleic acid YNB Oleic Acid -0.0 -0.3 N.D. +0.2 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 +0.0 N.D. +0.0 +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.2 N.D. -0.3 +0.6
growth on ypd YPD +0.1 +0.0 N.D. +0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.3 N.D. -0.0 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -0.1 N.D. +0.1 +1.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.1 N.D. +0.2 +0.5
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +1.0 N.D. +0.2 -0.1
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