Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15748 and 15749 are separated by 1050 nucleotides15749 and 15750 are separated by 47 nucleotides15750 and 15751 are separated by 694 nucleotides15751 and 15752 are separated by 606 nucleotides mRNA_7380: 15748 - K01897 ACSL, fadD long-chain acyl-CoA synthetase, at 136,989 to 140,179 15748 mRNA_7381: 15749 - K03128 TAF2 transcription initiation factor TFIID subunit 2, at 141,230 to 147,514 15749 mRNA_7382: 15750 - Hypothetical Protein, at 147,562 to 148,955 15750 mRNA_7383: 15751 - K19355 MAN mannan endo-1,4-beta-mannosidase, at 149,650 to 152,306 15751 mRNA_7384: 15752 - K11128 GAR1, NOLA1 H/ACA ribonucleoprotein complex subunit 1, at 152,913 to 154,132 15752
Group Condition mRNA_7380 mRNA_7381 mRNA_7382 mRNA_7383 mRNA_7384
15748 15749 15750 15751 15752
facs on lipid accumulation Sorted Low BODIPY Gate -1.5 N.D. +0.2 -2.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.8 N.D. -0.3 -1.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -1.1 N.D. -0.1 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.2 N.D. +0.4 -0.5 N.D.
growth on oleic acid YNB Glucose -1.6 N.D. +0.2 +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine -1.2 N.D. +0.3 +0.0 N.D.
auxotrophy YNB Glucose -1.0 N.D. +0.1 +0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine -1.3 N.D. +0.3 +0.2 N.D.
growth on ricinoleic acid YNB Glucose -1.2 N.D. +0.1 +0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.4 N.D. -0.3 -0.6 N.D.
auxotrophy Dropout Complete -0.8 N.D. +0.1 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. +0.0 +0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -1.2 N.D. +0.1 +1.1 N.D.
growth on ypd YPD +0.0 N.D. +0.3 -0.1 N.D.
growth on oleic acid YNB Oleic Acid -0.3 N.D. +0.2 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. +0.0 +0.3 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.3 N.D. -0.0 +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. -0.3 +0.6 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.2 +0.5 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. +0.1 +1.0 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +1.0 N.D. +0.2 -0.1 N.D.
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