Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15766 and 15767 are separated by 283 nucleotides15767 and 15768 are separated by 163 nucleotides15768 and 15769 are separated by 478 nucleotides15769 and 15770 are separated by 132 nucleotides mRNA_7398: 15766 - KOG0266 WD40 repeat-containing protein, at 184,149 to 185,888 15766 mRNA_7399: 15767 - K12859 TXNL4A, DIB1 U5 snRNP protein, DIM1 family, at 186,172 to 187,240 15767 mRNA_7400: 15768 - K08075 E3.1.3.32 polynucleotide 3'-phosphatase, at 187,404 to 189,530 15768 mRNA_7401: 15769 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 190,009 to 193,201 15769 mRNA_7402: 15770 - HMMPfam-Thaumatin family-PF00314,PRINTS-Pathogenesis-related protein signature-PR00347,ProSiteProfiles-Thaumatin family profile.-PS51367,SMART-Thaumatin family-SM00205,SUPERFAMILY--SSF49870, at 193,334 to 195,902 15770
Group Condition mRNA_7398 mRNA_7399 mRNA_7400 mRNA_7401 mRNA_7402
15766 15767 15768 15769 15770
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. -0.2 -0.2 -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. -0.3 -0.2 -0.7
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. -0.3 -0.1 -0.8
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. -0.3 -0.3 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 N.D. -0.0 -0.3 -0.8
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. -0.4 +0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. -0.3 +0.0 -0.2
growth on ypd YPD -0.1 N.D. -0.0 -0.3 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. -0.1 -0.0 -0.5
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. +0.0 -0.1 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. +0.2 +0.1 -0.4
auxotrophy Dropout Complete -0.1 N.D. -0.1 -0.0 +0.0
auxotrophy YNB Glucose -0.0 N.D. +0.1 +0.1 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 N.D. -0.4 +0.2 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. -0.3 +0.2 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. -0.2 +0.1 +0.1
growth on ricinoleic acid YNB Glucose +0.1 N.D. -0.3 +0.5 +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.2 +0.1 -0.1
growth on oleic acid YNB Glucose -0.0 N.D. +0.2 +0.1 +0.1
growth on oleic acid YNB Oleic Acid -0.2 N.D. +0.2 +0.4 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 N.D. +0.4 +0.3 -0.2
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