Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15779 and 15780 are separated by 207 nucleotides15780 and 15781 are separated by 120 nucleotides15781 and 15782 are separated by 258 nucleotides15782 and 15783 are separated by 188 nucleotides mRNA_7411: 15779 - K02537 MAD2 mitotic spindle assembly checkpoint protein MAD2, at 216,735 to 217,924 15779 mRNA_7412: 15780 - K03596 lepA GTP-binding protein LepA, at 218,132 to 221,123 15780 mRNA_7413: 15781 - K17550 PPP1R7, SDS22 protein phosphatase 1 regulatory subunit 7, at 221,244 to 223,051 15781 mRNA_7414: 15782 - Hypothetical Protein, at 223,310 to 224,480 15782 mRNA_7415: 15783 - K00623 CHAT choline O-acetyltransferase, at 224,669 to 227,280 15783
Group Condition mRNA_7411 mRNA_7412 mRNA_7413 mRNA_7414 mRNA_7415
15779 15780 15781 15782 15783
facs on lipid accumulation Sorted Low BODIPY Gate -2.9 +0.1 N.D. -0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -1.3 -0.2 N.D. -0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -1.5 +0.1 N.D. -0.0 N.D.
auxotrophy Dropout Complete -1.1 -0.1 N.D. +0.3 N.D.
growth on ypd YPD -0.6 -0.3 N.D. +0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.3 -0.2 N.D. -0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.7 +0.1 N.D. +0.0 N.D.
growth on oleic acid YNB Oleic Acid -0.0 -0.3 N.D. -0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 -0.4 N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 -0.1 N.D. +0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 -0.1 N.D. -0.0 N.D.
auxotrophy YNB Glucose -0.5 +0.1 N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.0 N.D. -0.2 N.D.
growth on oleic acid YNB Glucose +0.1 -0.1 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.1 N.D. -0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.5 +0.1 N.D. +0.5 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.0 N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.0 N.D. +0.2 N.D.
growth on ricinoleic acid YNB Glucose +0.0 +0.3 N.D. +0.3 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.0 N.D. +0.5 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.6 +0.0 N.D. +0.3 N.D.
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