Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15780 and 15781 are separated by 120 nucleotides15781 and 15782 are separated by 258 nucleotides15782 and 15783 are separated by 188 nucleotides15783 and 15784 are separated by 354 nucleotides mRNA_7412: 15780 - K03596 lepA GTP-binding protein LepA, at 218,132 to 221,123 15780 mRNA_7413: 15781 - K17550 PPP1R7, SDS22 protein phosphatase 1 regulatory subunit 7, at 221,244 to 223,051 15781 mRNA_7414: 15782 - Hypothetical Protein, at 223,310 to 224,480 15782 mRNA_7415: 15783 - K00623 CHAT choline O-acetyltransferase, at 224,669 to 227,280 15783 mRNA_7416: 15784 - Hypothetical Protein, at 227,635 to 230,239 15784
Group Condition mRNA_7412 mRNA_7413 mRNA_7414 mRNA_7415 mRNA_7416
15780 15781 15782 15783 15784
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. +0.5 N.D. -2.6
growth on oleic acid YNB Oleic Acid -0.3 N.D. -0.1 N.D. -1.5
auxotrophy Dropout Complete -0.1 N.D. +0.3 N.D. -1.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 N.D. +0.0 N.D. -0.6
growth on ricinoleic acid YNB Glucose +0.3 N.D. +0.3 N.D. -1.3
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. -0.0 N.D. -0.6
growth on ypd YPD -0.3 N.D. +0.2 N.D. -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. +0.2 N.D. -0.9
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 N.D. +0.3 N.D. -0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. -0.0 N.D. -0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. -0.1 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. +0.0 N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. -0.2 N.D. +0.0
growth on oleic acid YNB Glucose -0.1 N.D. -0.2 N.D. +0.1
auxotrophy YNB Glucose +0.1 N.D. +0.1 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. -0.1 N.D. +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. +0.2 N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. -0.2 N.D. +0.4
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. +0.5 N.D. +0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. -0.0 N.D. +1.2
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. +0.3 N.D. +1.0
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