Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15805 and 15806 are separated by 253 nucleotides15806 and 15807 are separated by 926 nucleotides15807 and 15808 are separated by 240 nucleotides15808 and 15809 are separated by 1727 nucleotides mRNA_7437: 15805 - K01756 purB, ADSL adenylosuccinate lyase, at 276,524 to 278,511 15805 mRNA_7438: 15806 - K01455 E3.5.1.49 formamidase, at 278,765 to 280,421 15806 mRNA_7439: 15807 - K20989 DUR3 urea-proton symporter, at 281,348 to 284,451 15807 mRNA_7440: 15808 - KOG0537 Cytochrome b5, at 284,692 to 285,285 15808 mRNA_7441: 15809 - K08884 K08884 serine/threonine protein kinase, bacterial, at 287,013 to 288,261 15809
Group Condition mRNA_7437 mRNA_7438 mRNA_7439 mRNA_7440 mRNA_7441
15805 15806 15807 15808 15809
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 +0.1 -0.7 -0.8
growth on oleic acid YNB Oleic Acid N.D. +0.1 +0.1 -0.2 -0.6
growth on ypd YPD N.D. +0.4 -0.0 -0.4 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 -0.3 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 -0.1 +0.0 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.3 -0.1 -0.4 +0.2
auxotrophy Dropout Complete N.D. -0.1 -0.1 -0.4 +0.5
growth on oleic acid YNB Glucose N.D. -0.0 +0.2 +0.1 -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 -0.5 +0.0 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. -0.0 -0.0 -0.2 +0.7
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.3 -0.0 +0.1 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +1.0 +0.1 -0.4 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.0 +0.1 +0.4 +0.2
auxotrophy YNB Glucose N.D. -0.1 +0.0 -0.1 +0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.3 +0.0 +0.2 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 -0.1 -0.3 +1.5
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 -0.0 -0.4 +1.2
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 -0.0 -0.2 +1.6
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 +0.1 -0.1 +1.4
growth on ricinoleic acid YNB Glucose N.D. +0.6 +0.0 -0.2 +1.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.9 +0.1 -0.2 +1.0
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